Details
| Original language | English |
|---|---|
| Title of host publication | Optogenetics and Optical Manipulation 2020 |
| Publisher | SPIE |
| ISBN (electronic) | 9781510632172 |
| Publication status | Published - 19 Feb 2020 |
| Event | Optogenetics and Optical Manipulation 2020 - San Francisco, United States Duration: 1 Feb 2020 → 2 Feb 2020 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 11227 |
| ISSN (Print) | 1605-7422 |
Abstract
In the past decade, in vivo models for optogenetic applications have gained importance, especially in the fields of cardiology and neuroscience. To reliably evoke the desired reactions while minimizing adverse effects, the stimulation power must be carefully adjusted. The relevant light intensity for cells in deeper layers of scattering tissue is not easily extrapolated from the power threshold of single cells. In this study, we evaluated a model for deep tissue optogenetic stimulation, using a heart-like cell line and tissue phantoms. Phantoms were fabricated from PDMS and titanium dioxide particles and possessed highly reproducible optical properties. Scattering and absorption coefficients were modeled to match those of realistic tissues. Since power of light traveling through tissues decays exponentially with respect to the scattering and absorption coefficients, the required input power was expected to increase exponentially by the same factor. To test this hypothesis, cells were stimulated through tissue phantoms of varying thickness with different modes of illumination. Cellular reactions revealed that the simplified assumptions were not sufficient to predict the input power required to reach the stimulation threshold. We provide a more comprehensive model to assess cellular reactions in scattering tissues a priori. This study has implications for the use of optogenetics in tissue models, organs and in vivo models as the outcomes can be transferred to different types of cells and tissues.
Keywords
- Light diffusion, Optogenetics, Tissue phantoms
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Optogenetics and Optical Manipulation 2020. SPIE, 2020. 1122708 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11227).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Evaluation of a model for deep tissue optogenetic stimulation
AU - Johannsmeier, Sonja
AU - Wenzel, Johannes
AU - Torres-Mapa, Maria L.
AU - Junge, Sebastian
AU - Sasse, Philipp
AU - Ripken, Tammo
AU - Heinemann, Dag
AU - Heisterkamp, Alexander
N1 - Funding information: This work is funded by the Federal Ministry of Education and Research, Germany, Grant no. 13N14085 and supported by the DFG Cluster of Excellence “Hearing4all” (EXC 1077/1).
PY - 2020/2/19
Y1 - 2020/2/19
N2 - In the past decade, in vivo models for optogenetic applications have gained importance, especially in the fields of cardiology and neuroscience. To reliably evoke the desired reactions while minimizing adverse effects, the stimulation power must be carefully adjusted. The relevant light intensity for cells in deeper layers of scattering tissue is not easily extrapolated from the power threshold of single cells. In this study, we evaluated a model for deep tissue optogenetic stimulation, using a heart-like cell line and tissue phantoms. Phantoms were fabricated from PDMS and titanium dioxide particles and possessed highly reproducible optical properties. Scattering and absorption coefficients were modeled to match those of realistic tissues. Since power of light traveling through tissues decays exponentially with respect to the scattering and absorption coefficients, the required input power was expected to increase exponentially by the same factor. To test this hypothesis, cells were stimulated through tissue phantoms of varying thickness with different modes of illumination. Cellular reactions revealed that the simplified assumptions were not sufficient to predict the input power required to reach the stimulation threshold. We provide a more comprehensive model to assess cellular reactions in scattering tissues a priori. This study has implications for the use of optogenetics in tissue models, organs and in vivo models as the outcomes can be transferred to different types of cells and tissues.
AB - In the past decade, in vivo models for optogenetic applications have gained importance, especially in the fields of cardiology and neuroscience. To reliably evoke the desired reactions while minimizing adverse effects, the stimulation power must be carefully adjusted. The relevant light intensity for cells in deeper layers of scattering tissue is not easily extrapolated from the power threshold of single cells. In this study, we evaluated a model for deep tissue optogenetic stimulation, using a heart-like cell line and tissue phantoms. Phantoms were fabricated from PDMS and titanium dioxide particles and possessed highly reproducible optical properties. Scattering and absorption coefficients were modeled to match those of realistic tissues. Since power of light traveling through tissues decays exponentially with respect to the scattering and absorption coefficients, the required input power was expected to increase exponentially by the same factor. To test this hypothesis, cells were stimulated through tissue phantoms of varying thickness with different modes of illumination. Cellular reactions revealed that the simplified assumptions were not sufficient to predict the input power required to reach the stimulation threshold. We provide a more comprehensive model to assess cellular reactions in scattering tissues a priori. This study has implications for the use of optogenetics in tissue models, organs and in vivo models as the outcomes can be transferred to different types of cells and tissues.
KW - Light diffusion
KW - Optogenetics
KW - Tissue phantoms
UR - http://www.scopus.com/inward/record.url?scp=85082501742&partnerID=8YFLogxK
U2 - 10.1117/12.2544837
DO - 10.1117/12.2544837
M3 - Conference contribution
AN - SCOPUS:85082501742
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optogenetics and Optical Manipulation 2020
PB - SPIE
T2 - Optogenetics and Optical Manipulation 2020
Y2 - 1 February 2020 through 2 February 2020
ER -