Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Publisher | International Society for Horticultural Science |
| Pages | 239-242 |
| Number of pages | 4 |
| ISBN (print) | 9789066051102 |
| Publication status | Published - 28 Feb 2010 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 855 |
| ISSN (Print) | 0567-7572 |
Abstract
Comprehensive proteomic characterizations were performed aiming to create proteome reference maps for somatic and zygotic embryos of Cyclamen persicum. Separation by two dimensional isoelectric focusing - sodium dodecyl sulfate polyacrylamide gel electrophoresis led to a resolution of more than 800 protein spots for each tissue. Approximately 70% of the spots likewise appeared in both zygotic and in somatic embryo's protein fractions. However, differential gel electrophoresis analyses revealed pronounced differences in abundances for the majority of proteins present in both tissues. MS analyses for 300 reproducible spots in total (263 of the zygotic embryos' protein fraction and 37 spots appearing specifically in the somatic embryos' proteome) led to identification of 261 proteins, 35 of which were specific or highly abundant in gels of the somatic embryo's tissue. Most identified proteins were found to be involved in glycolysis or gluconeogenesis and stress response pathways.
Keywords
- Differential gel electrophoresis, Embryogenesis, Mass spectrometry, Protein identification, Two-dimensional polyacrylamide gel electrophoresis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. International Society for Horticultural Science, 2010. p. 239-242 (Acta Horticulturae; Vol. 855).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Establishment of Proteome Reference Maps for Somatic and Zygotic Embryos of Cyclamen persicum Mill
AU - Rode, C.
AU - Braun, H. P.
AU - Gallien, S.
AU - Heintz, D.
AU - Van Dorsselaer, A.
AU - Winkelmann, T.
PY - 2010/2/28
Y1 - 2010/2/28
N2 - Comprehensive proteomic characterizations were performed aiming to create proteome reference maps for somatic and zygotic embryos of Cyclamen persicum. Separation by two dimensional isoelectric focusing - sodium dodecyl sulfate polyacrylamide gel electrophoresis led to a resolution of more than 800 protein spots for each tissue. Approximately 70% of the spots likewise appeared in both zygotic and in somatic embryo's protein fractions. However, differential gel electrophoresis analyses revealed pronounced differences in abundances for the majority of proteins present in both tissues. MS analyses for 300 reproducible spots in total (263 of the zygotic embryos' protein fraction and 37 spots appearing specifically in the somatic embryos' proteome) led to identification of 261 proteins, 35 of which were specific or highly abundant in gels of the somatic embryo's tissue. Most identified proteins were found to be involved in glycolysis or gluconeogenesis and stress response pathways.
AB - Comprehensive proteomic characterizations were performed aiming to create proteome reference maps for somatic and zygotic embryos of Cyclamen persicum. Separation by two dimensional isoelectric focusing - sodium dodecyl sulfate polyacrylamide gel electrophoresis led to a resolution of more than 800 protein spots for each tissue. Approximately 70% of the spots likewise appeared in both zygotic and in somatic embryo's protein fractions. However, differential gel electrophoresis analyses revealed pronounced differences in abundances for the majority of proteins present in both tissues. MS analyses for 300 reproducible spots in total (263 of the zygotic embryos' protein fraction and 37 spots appearing specifically in the somatic embryos' proteome) led to identification of 261 proteins, 35 of which were specific or highly abundant in gels of the somatic embryo's tissue. Most identified proteins were found to be involved in glycolysis or gluconeogenesis and stress response pathways.
KW - Differential gel electrophoresis
KW - Embryogenesis
KW - Mass spectrometry
KW - Protein identification
KW - Two-dimensional polyacrylamide gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=77952678567&partnerID=8YFLogxK
U2 - 10.15488/11661
DO - 10.15488/11661
M3 - Conference contribution
AN - SCOPUS:77952678567
SN - 9789066051102
T3 - Acta Horticulturae
SP - 239
EP - 242
BT - Acta Horticulturae
PB - International Society for Horticultural Science
ER -