Details
| Original language | English |
|---|---|
| Title of host publication | I International Orchid Symposium |
| Publisher | International Society for Horticultural Science |
| Pages | 445-452 |
| Number of pages | 8 |
| ISBN (print) | 9789066055735 |
| Publication status | Published - 29 Oct 2010 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 878 |
| ISSN (Print) | 0567-7572 |
Abstract
Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance. The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis. Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar 'SP 12' and Sharry Baby var. aureum 'OK', as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria 'Plush', we established a reliable protocol for regeneration of somatic embryos. The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks. The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
Keywords
- Embryogenesis, Liquid culture, Micropropagation, Orchids, Somatic, Thidiazuron
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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I International Orchid Symposium. International Society for Horticultural Science, 2010. p. 445-452 (Acta Horticulturae; Vol. 878).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Establishment and optimization of an efficient in vitro regeneration system of oncidium, wilsonara, odontocidium and vuylstekeara
AU - Raffeiner, B.
AU - Serek, M.
AU - Winkelmann, T.
PY - 2010/10/29
Y1 - 2010/10/29
N2 - Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance. The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis. Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar 'SP 12' and Sharry Baby var. aureum 'OK', as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria 'Plush', we established a reliable protocol for regeneration of somatic embryos. The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks. The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
AB - Hybrids of Oncidium and Odontoglossum are orchids with a wide spectrum of flower colors and patterns and also with increasing economic importance. The objective of this study was to develop an efficient protocol for in vitro regeneration via somatic embryogenesis. Starting from in vitro shoot cultures of the cultivars Oncidium Sweet Sugar 'SP 12' and Sharry Baby var. aureum 'OK', as well as the hybrids Wilsonara Stirling Tiger, Odontocidium Hansueli Isler and Vuylstekeara Cambria 'Plush', we established a reliable protocol for regeneration of somatic embryos. The most suitable type of explants were leaf tips, on which the formation of protocorm-like bodies was observed in frequencies of 60% after 12 weeks. The most efficient regeneration was obtained on medium containing 1 mg/L thidiazuron and cultured in complete darkness.
KW - Embryogenesis
KW - Liquid culture
KW - Micropropagation
KW - Orchids
KW - Somatic
KW - Thidiazuron
UR - http://www.scopus.com/inward/record.url?scp=80053128145&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2010.878.57
DO - 10.17660/ActaHortic.2010.878.57
M3 - Conference contribution
AN - SCOPUS:80053128145
SN - 9789066055735
T3 - Acta Horticulturae
SP - 445
EP - 452
BT - I International Orchid Symposium
PB - International Society for Horticultural Science
ER -