Details
| Original language | English |
|---|---|
| Pages (from-to) | 3657-3664 |
| Number of pages | 8 |
| Journal | Organic and Biomolecular Chemistry |
| Volume | 5 |
| Issue number | 22 |
| Publication status | Published - 2007 |
Abstract
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-d-glucal (17) in a flow-through mode.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
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In: Organic and Biomolecular Chemistry, Vol. 5, No. 22, 2007, p. 3657-3664.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix
AU - Dräger, Gerald
AU - Kiss, Csilla
AU - Kunz, Ulrich
AU - Kirschning, Andreas
PY - 2007
Y1 - 2007
N2 - The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-d-glucal (17) in a flow-through mode.
AB - The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His6-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-d-glucal (17) in a flow-through mode.
UR - http://www.scopus.com/inward/record.url?scp=35649028416&partnerID=8YFLogxK
U2 - 10.1039/b712804e
DO - 10.1039/b712804e
M3 - Article
C2 - 17971995
AN - SCOPUS:35649028416
VL - 5
SP - 3657
EP - 3664
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
SN - 1477-0520
IS - 22
ER -