Details
| Original language | English |
|---|---|
| Title of host publication | Deep Eutectic Solvents |
| Subtitle of host publication | Synthesis, Properties, and Applications |
| Publisher | Wiley-VCH Verlag |
| Pages | 257-271 |
| Number of pages | 15 |
| ISBN (electronic) | 9783527818471 |
| ISBN (print) | 9783527345182 |
| Publication status | Published - 25 Nov 2019 |
| Externally published | Yes |
Abstract
Deep eutectic solvents (DESs) have been studied for biocatalysis with hydrolases, oxidoreductases, laccases, lyases, etc., using free enzymes or whole cells. DESs can be used as solvents for biotransformations, enabling the dissolution of substrates with different polarities, opening options for challenging reactions. The addition of water as cosolvent up to 20% (v/v) leads to DES-water mixtures with diminished viscosity but remaining as nonaqueous solvent. For enzyme catalysis, DES can also be used as cosolvents, when water is added in larger amounts (≥20% v/v). In these cases, improvements in enzyme stability and selectivity have been described. Finally, DES can be used as media for multistep catalytic processes, as well as for end-of-pipe tools to separate products and substrates efficiently, after an enzymatic transformation. Overall, DESs represent solid, sustainable, and promising alternatives for biocatalysis.
Keywords
- Biocatalysis, Deep eutectic solvents, Downstream processing, Lipases, Oxidoreductases, Whole cells
ASJC Scopus subject areas
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Deep Eutectic Solvents: Synthesis, Properties, and Applications. Wiley-VCH Verlag, 2019. p. 257-271.
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Enzyme catalysis
T2 - In DES, with DES, and in the presence of DES
AU - De María, Pablo Domínguez
AU - Guajardo, Nadia
AU - Kara, Selin
PY - 2019/11/25
Y1 - 2019/11/25
N2 - Deep eutectic solvents (DESs) have been studied for biocatalysis with hydrolases, oxidoreductases, laccases, lyases, etc., using free enzymes or whole cells. DESs can be used as solvents for biotransformations, enabling the dissolution of substrates with different polarities, opening options for challenging reactions. The addition of water as cosolvent up to 20% (v/v) leads to DES-water mixtures with diminished viscosity but remaining as nonaqueous solvent. For enzyme catalysis, DES can also be used as cosolvents, when water is added in larger amounts (≥20% v/v). In these cases, improvements in enzyme stability and selectivity have been described. Finally, DES can be used as media for multistep catalytic processes, as well as for end-of-pipe tools to separate products and substrates efficiently, after an enzymatic transformation. Overall, DESs represent solid, sustainable, and promising alternatives for biocatalysis.
AB - Deep eutectic solvents (DESs) have been studied for biocatalysis with hydrolases, oxidoreductases, laccases, lyases, etc., using free enzymes or whole cells. DESs can be used as solvents for biotransformations, enabling the dissolution of substrates with different polarities, opening options for challenging reactions. The addition of water as cosolvent up to 20% (v/v) leads to DES-water mixtures with diminished viscosity but remaining as nonaqueous solvent. For enzyme catalysis, DES can also be used as cosolvents, when water is added in larger amounts (≥20% v/v). In these cases, improvements in enzyme stability and selectivity have been described. Finally, DES can be used as media for multistep catalytic processes, as well as for end-of-pipe tools to separate products and substrates efficiently, after an enzymatic transformation. Overall, DESs represent solid, sustainable, and promising alternatives for biocatalysis.
KW - Biocatalysis
KW - Deep eutectic solvents
KW - Downstream processing
KW - Lipases
KW - Oxidoreductases
KW - Whole cells
UR - http://www.scopus.com/inward/record.url?scp=85100264661&partnerID=8YFLogxK
U2 - 10.1002/9783527818488.ch13
DO - 10.1002/9783527818488.ch13
M3 - Contribution to book/anthology
AN - SCOPUS:85100264661
SN - 9783527345182
SP - 257
EP - 271
BT - Deep Eutectic Solvents
PB - Wiley-VCH Verlag
ER -