Details
| Original language | English |
|---|---|
| Pages (from-to) | 1104-1111 |
| Number of pages | 8 |
| Journal | International Journal of Phytoremediation |
| Volume | 21 |
| Issue number | 11 |
| Early online date | 30 Apr 2019 |
| Publication status | Published - 19 Sept 2019 |
Abstract
The biodegradation of the antibiotic sulfamethazine (SMT) by using different crude extracts of halophytes was investigated. For this purpose, crude water extracts of the halophytes Chenopodium quinoa, Sesuvium portulacastrum, and Tripolium pannonicum were prepared. Different amounts of SMT were added to the different extracts (final concentration of 1, 2, and 5 mg L−1) and incubated at 37 °C. Crude extracts of T. pannonicum were further used to evaluate the degradation rate over time. In order to evaluate the influence of endophytic or naturally plant-associated microorganisms on the biodegradation of SMT, extracts from plants grown in sterile and non-sterile conditions were compared. SMT was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, crude extracts of T. pannonicum have a high potential to biodegrade SMT with a decrease up to 85.4% (4.27 ± 0.10 mg L−1) from an initial concentration of 5 mg L−1. The lowest activity was obtained using extracts of C. quinoa with degradation of 4.5%. Extracts of plants cultivated under sterile and non-sterile conditions do not have any significant difference in SMT degradation. Therefore, microorganisms and their enzymatic activities do not seem to play a significant role during this process.
Keywords
- Enzymatic assay, halophyte plants, phytoremediation, sulfamethazine biodegradation
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Pollution
- Agricultural and Biological Sciences(all)
- Plant Science
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In: International Journal of Phytoremediation, Vol. 21, No. 11, 19.09.2019, p. 1104-1111.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enzymatic degradation of the antibiotic sulfamethazine by using crude extracts of different halophytic plants
AU - Turcios Pantaleón, Ariel Eliseo
AU - Papenbrock, Jutta
N1 - We would like to thank Michael Werner for his support in the lab, Yvonne Leye and Fabian Söffker for taking care of the plants.
PY - 2019/9/19
Y1 - 2019/9/19
N2 - The biodegradation of the antibiotic sulfamethazine (SMT) by using different crude extracts of halophytes was investigated. For this purpose, crude water extracts of the halophytes Chenopodium quinoa, Sesuvium portulacastrum, and Tripolium pannonicum were prepared. Different amounts of SMT were added to the different extracts (final concentration of 1, 2, and 5 mg L−1) and incubated at 37 °C. Crude extracts of T. pannonicum were further used to evaluate the degradation rate over time. In order to evaluate the influence of endophytic or naturally plant-associated microorganisms on the biodegradation of SMT, extracts from plants grown in sterile and non-sterile conditions were compared. SMT was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, crude extracts of T. pannonicum have a high potential to biodegrade SMT with a decrease up to 85.4% (4.27 ± 0.10 mg L−1) from an initial concentration of 5 mg L−1. The lowest activity was obtained using extracts of C. quinoa with degradation of 4.5%. Extracts of plants cultivated under sterile and non-sterile conditions do not have any significant difference in SMT degradation. Therefore, microorganisms and their enzymatic activities do not seem to play a significant role during this process.
AB - The biodegradation of the antibiotic sulfamethazine (SMT) by using different crude extracts of halophytes was investigated. For this purpose, crude water extracts of the halophytes Chenopodium quinoa, Sesuvium portulacastrum, and Tripolium pannonicum were prepared. Different amounts of SMT were added to the different extracts (final concentration of 1, 2, and 5 mg L−1) and incubated at 37 °C. Crude extracts of T. pannonicum were further used to evaluate the degradation rate over time. In order to evaluate the influence of endophytic or naturally plant-associated microorganisms on the biodegradation of SMT, extracts from plants grown in sterile and non-sterile conditions were compared. SMT was analyzed by liquid chromatography coupled to positive ion electrospray mass spectrometry (ESI LC-MS). Based on the findings, crude extracts of T. pannonicum have a high potential to biodegrade SMT with a decrease up to 85.4% (4.27 ± 0.10 mg L−1) from an initial concentration of 5 mg L−1. The lowest activity was obtained using extracts of C. quinoa with degradation of 4.5%. Extracts of plants cultivated under sterile and non-sterile conditions do not have any significant difference in SMT degradation. Therefore, microorganisms and their enzymatic activities do not seem to play a significant role during this process.
KW - Enzymatic assay
KW - halophyte plants
KW - phytoremediation
KW - sulfamethazine biodegradation
UR - http://www.scopus.com/inward/record.url?scp=85065240043&partnerID=8YFLogxK
U2 - 10.1080/15226514.2019.1606782
DO - 10.1080/15226514.2019.1606782
M3 - Article
C2 - 31037959
AN - SCOPUS:85065240043
VL - 21
SP - 1104
EP - 1111
JO - International Journal of Phytoremediation
JF - International Journal of Phytoremediation
SN - 1522-6514
IS - 11
ER -