Details
| Translated title of the contribution | Enzymatische Umsetzung von Flavonoiden mit einer bakteriellen Chalconisomerase und einer Enoatreduktase |
|---|---|
| Original language | English |
| Pages (from-to) | 1439-1442 |
| Number of pages | 4 |
| Journal | Angewandte Chemie |
| Volume | 53 |
| Issue number | 5 |
| Publication status | Published - 20 Dec 2013 |
Abstract
Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
Keywords
- Chalcone isomerase, Enoate reductase, Enzyme biocatalysis, Eubacterium ramulus, Flavonoids
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
- General Chemistry
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In: Angewandte Chemie , Vol. 53, No. 5, 20.12.2013, p. 1439-1442.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enzymatic Conversion of Flavonoids using Bacterial ChalconeIsomerase and Enoate Reductase
AU - Gall, Mechthild
AU - Thomsen, Maren
AU - Peters, Christin
AU - Pavlidis, Ioannis V.
AU - Jonczyk, Patrick
AU - Grunert, Philipp P.
AU - Beutel, Sascha
AU - Scheper, Thomas
AU - Gross, Egon
AU - Backes, Michael
AU - Geißler, Torsten
AU - Ley, Jakob P.
AU - Hilmer, Jens Michael
AU - Krammer, Gerhard
AU - Palm, Gottfried J.
AU - Hinrichs, Winfried
AU - Bornscheuer, Uwe T.
PY - 2013/12/20
Y1 - 2013/12/20
N2 - Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
AB - Flavonoids are a large group of plant secondary metabolites with a variety of biological properties and are therefore of interest to many scientists, as they can lead to industrially interesting intermediates. The anaerobic gut bacterium Eubacterium ramulus can catabolize flavonoids, but until now, the pathway has not been experimentally confirmed. In the present work, a chalcone isomerase (CHI) and an enoate reductase (ERED) could be identified through whole genome sequencing and gene motif search. These two enzymes were successfully cloned and expressed in Escherichia coli in their active form, even under aerobic conditions. The catabolic pathway of E. ramulus was confirmed by biotransformations of flavanones into dihydrochalcones. The engineered E. coli strain that expresses both enzymes was used for the conversion of several flavanones, underlining the applicability of this biocatalytic cascade reaction.
KW - Chalcone isomerase
KW - Enoate reductase
KW - Enzyme biocatalysis
KW - Eubacterium ramulus
KW - Flavonoids
UR - http://www.scopus.com/inward/record.url?scp=84893976023&partnerID=8YFLogxK
U2 - 10.1002/anie.201306952
DO - 10.1002/anie.201306952
M3 - Article
C2 - 24459060
AN - SCOPUS:84893976023
VL - 53
SP - 1439
EP - 1442
JO - Angewandte Chemie
JF - Angewandte Chemie
SN - 1433-7851
IS - 5
ER -