Details
| Original language | English |
|---|---|
| Pages (from-to) | 474-482 |
| Number of pages | 9 |
| Journal | Journal of Biophotonics |
| Volume | 7 |
| Issue number | 7 |
| Publication status | Published - 22 Jan 2013 |
| Externally published | Yes |
Abstract
The use of laser induced surface plasmons on metal nanoparticles has proven to be an excellent tool for the delivery of molecules like siRNA and DNA into cells. However, a detailed understanding of the basic mechanisms of molecular uptake and the influence of parameters like biological environment is missing. In this study we analyzed the uptake of fluorescent dextrans with sizes from 10 to 2000 kDa, which resembles a wide range of biologically relevant molecules in size using a 532 nm picosecond laser system and 200 nm gold nanoparticles. Our results show a strong uptake-dependence on cell medium or buffer, but no dominant dependence on osmotic conditions. The relation between pulse energy and number of pulses for a given perforation efficiency revealed that multiphoton ionization of water might contribute to perforation. Moreover, a seven-fold uptake-enhancement could be reached with optimized parameters, providing a very promising basis for further studies and applications.
Keywords
- Gene transfection, Multiphoton processes, Optoinjection, Photoporation
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Journal of Biophotonics, Vol. 7, No. 7, 22.01.2013, p. 474-482.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enhancement of extracellular molecule uptake in plasmonic laser perforation
AU - Kalies, Stefan
AU - Birr, Tobias
AU - Heinemann, Dag
AU - Schomaker, Markus
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
AU - Meyer, Heiko
PY - 2013/1/22
Y1 - 2013/1/22
N2 - The use of laser induced surface plasmons on metal nanoparticles has proven to be an excellent tool for the delivery of molecules like siRNA and DNA into cells. However, a detailed understanding of the basic mechanisms of molecular uptake and the influence of parameters like biological environment is missing. In this study we analyzed the uptake of fluorescent dextrans with sizes from 10 to 2000 kDa, which resembles a wide range of biologically relevant molecules in size using a 532 nm picosecond laser system and 200 nm gold nanoparticles. Our results show a strong uptake-dependence on cell medium or buffer, but no dominant dependence on osmotic conditions. The relation between pulse energy and number of pulses for a given perforation efficiency revealed that multiphoton ionization of water might contribute to perforation. Moreover, a seven-fold uptake-enhancement could be reached with optimized parameters, providing a very promising basis for further studies and applications.
AB - The use of laser induced surface plasmons on metal nanoparticles has proven to be an excellent tool for the delivery of molecules like siRNA and DNA into cells. However, a detailed understanding of the basic mechanisms of molecular uptake and the influence of parameters like biological environment is missing. In this study we analyzed the uptake of fluorescent dextrans with sizes from 10 to 2000 kDa, which resembles a wide range of biologically relevant molecules in size using a 532 nm picosecond laser system and 200 nm gold nanoparticles. Our results show a strong uptake-dependence on cell medium or buffer, but no dominant dependence on osmotic conditions. The relation between pulse energy and number of pulses for a given perforation efficiency revealed that multiphoton ionization of water might contribute to perforation. Moreover, a seven-fold uptake-enhancement could be reached with optimized parameters, providing a very promising basis for further studies and applications.
KW - Gene transfection
KW - Multiphoton processes
KW - Optoinjection
KW - Photoporation
UR - http://www.scopus.com/inward/record.url?scp=84903721605&partnerID=8YFLogxK
U2 - 10.1002/jbio.201200200
DO - 10.1002/jbio.201200200
M3 - Article
C2 - 23341255
AN - SCOPUS:84903721605
VL - 7
SP - 474
EP - 482
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 7
ER -