Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding |
| Publisher | International Society for Horticultural Science |
| Pages | 127-130 |
| Number of pages | 4 |
| ISBN (print) | 906605719X, 9789066057197 |
| Publication status | Published - Nov 2006 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 725 I |
| ISSN (Print) | 0567-7572 |
Abstract
Several plant species are recalcitrant when considering regeneration through adventitious organs. Establishment of regeneration systems for the plants of interest is a pre-requisite for further work such as genetic transformation and induced mutation. The cactus plants belonging to species Schlumbergera and Rhipsalidopsis were found to be recalcitrant. In addition to this problem, they were very slow to grow. However, it was possible to achieve high regenerative ability in explants of in vitro cultures of these cacti. The initial phylloclade explants established from adult plants grown in greenhouse were found to be recalcitrant. These explants produced only axillary shoots from the pre-formed shoot primodia when treated with exogenous plant hormones, but they failed to produce any adventitious shoots. There was a significant improvement in adventitious shoot formation in phylloclade explants, more particularly, of Rhipsalidopsis in long-term cultures. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of one of the cultivars of Rhipsalidopsis produced adventitious shoots. There was an increase in the regenerative capacity in the explants of Schlumbergera, however, the efficiency was not to the same extent as for the other species. Callus cultures were found to be more regenerative compared to the phylloclade explants. The results clearly indicated that the in vitro manipulations helped in increasing the capacity to regenerate.
Keywords
- Adventitious shoots, Christmas cactus, Easter cactus
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding. International Society for Horticultural Science, 2006. p. 127-130 (Acta Horticulturae; Vol. 725 I).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Enhanced regenerative capacity in Rhipsalidopsis and Schlumbergera explants by in vitro manipulations
AU - Sriskandarajah, S.
AU - Al-Ramamneh, E.
AU - Serek, M.
PY - 2006/11
Y1 - 2006/11
N2 - Several plant species are recalcitrant when considering regeneration through adventitious organs. Establishment of regeneration systems for the plants of interest is a pre-requisite for further work such as genetic transformation and induced mutation. The cactus plants belonging to species Schlumbergera and Rhipsalidopsis were found to be recalcitrant. In addition to this problem, they were very slow to grow. However, it was possible to achieve high regenerative ability in explants of in vitro cultures of these cacti. The initial phylloclade explants established from adult plants grown in greenhouse were found to be recalcitrant. These explants produced only axillary shoots from the pre-formed shoot primodia when treated with exogenous plant hormones, but they failed to produce any adventitious shoots. There was a significant improvement in adventitious shoot formation in phylloclade explants, more particularly, of Rhipsalidopsis in long-term cultures. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of one of the cultivars of Rhipsalidopsis produced adventitious shoots. There was an increase in the regenerative capacity in the explants of Schlumbergera, however, the efficiency was not to the same extent as for the other species. Callus cultures were found to be more regenerative compared to the phylloclade explants. The results clearly indicated that the in vitro manipulations helped in increasing the capacity to regenerate.
AB - Several plant species are recalcitrant when considering regeneration through adventitious organs. Establishment of regeneration systems for the plants of interest is a pre-requisite for further work such as genetic transformation and induced mutation. The cactus plants belonging to species Schlumbergera and Rhipsalidopsis were found to be recalcitrant. In addition to this problem, they were very slow to grow. However, it was possible to achieve high regenerative ability in explants of in vitro cultures of these cacti. The initial phylloclade explants established from adult plants grown in greenhouse were found to be recalcitrant. These explants produced only axillary shoots from the pre-formed shoot primodia when treated with exogenous plant hormones, but they failed to produce any adventitious shoots. There was a significant improvement in adventitious shoot formation in phylloclade explants, more particularly, of Rhipsalidopsis in long-term cultures. After the first subculture, sporadic adventitious shoot formation began, and after the third subculture 87% explants of one of the cultivars of Rhipsalidopsis produced adventitious shoots. There was an increase in the regenerative capacity in the explants of Schlumbergera, however, the efficiency was not to the same extent as for the other species. Callus cultures were found to be more regenerative compared to the phylloclade explants. The results clearly indicated that the in vitro manipulations helped in increasing the capacity to regenerate.
KW - Adventitious shoots
KW - Christmas cactus
KW - Easter cactus
UR - http://www.scopus.com/inward/record.url?scp=33846469437&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2006.725.11
DO - 10.17660/ActaHortic.2006.725.11
M3 - Conference contribution
AN - SCOPUS:33846469437
SN - 906605719X
SN - 9789066057197
T3 - Acta Horticulturae
SP - 127
EP - 130
BT - Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding
PB - International Society for Horticultural Science
ER -