Details
| Original language | English |
|---|---|
| Pages (from-to) | 4500-4509 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 102 |
| Issue number | 12 |
| Early online date | 26 Feb 2014 |
| Publication status | Published - 1 Dec 2014 |
Abstract
Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.
Keywords
- Electrospinning, Endothelial cells, Graft, Tissue engineering, Vascular prosthesis
ASJC Scopus subject areas
- Materials Science(all)
- Ceramics and Composites
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Materials Science(all)
- Metals and Alloys
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In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 12, 01.12.2014, p. 4500-4509.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Endothelialization of electrospun polycaprolactone (PCL) small caliber vascular grafts spun from different polymer blends
AU - Pfeiffer, Dagmar
AU - Stefanitsch, C.
AU - Wankhammer, K.
AU - Müller, M.
AU - Dreyer, L.
AU - Krolitzki, B.
AU - Zernetsch, H.
AU - Glasmacher, B.
AU - Lindner, C.
AU - Lass, A.
AU - Schwarz, M.
AU - Muckenauer, W.
AU - Lang-Olip, Ingrid
N1 - German Research Foundation; contract grant number: SFB-TR37-Q2, B4 REBIRTH; contract grant number: EXC 62/1 Government of Styria; contract grant number: A3–22H-2/2011-41 Lower Austria; contract grant number: WST3-T-91/023–2010; LS09–018
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.
AB - Small caliber vascular grafts represent a challenge to material scientists. In contrast to large caliber grafts, prostheses with diameter <6 mm, lead to increased hemodynamic disturbances and thrombogenic complications. Thus, endothelialization of small caliber grafts should create a compatible interface for hemodynamic processes. The purpose of our study was to compare different compositions of electrospun scaffolds with conventional ePTFE grafts with an inner diameter of 4 mm as well as different pre-coatings to create an optimized physiological interface for endothelialization. Polycaprolactone, polylactide, and polyethylenglycol (PCL/PLA and PCL/PLA/PEG) electrospun grafts and ePTFE grafts were pre-coated with blood, gelatine or fibronectin and seeded with endothelial cells from the human term placenta. Best results were obtained with fibronectin-coated PCL/PLA/PEG grafts. Here, the number of attached viable cells was 78-81% higher than on fibronectin pre-treated ePTFE grafts. Cells attached to PCL/PLA/PEG grafts appeared in physiological cobblestone morphology. Viability analysis showed a high cell viability of more than 98%. Fibronectin-coated PCL/PLA/PEG grafts may be a promising improvement to conventionally used ePTFE grafts.
KW - Electrospinning
KW - Endothelial cells
KW - Graft
KW - Tissue engineering
KW - Vascular prosthesis
UR - http://www.scopus.com/inward/record.url?scp=84911806556&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.35123
DO - 10.1002/jbm.a.35123
M3 - Article
C2 - 24532056
AN - SCOPUS:84911806556
VL - 102
SP - 4500
EP - 4509
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 12
ER -