Details
| Original language | English |
|---|---|
| Article number | 1785 |
| Journal | Frontiers in Plant Science |
| Volume | 9 |
| Early online date | 6 Dec 2018 |
| Publication status | Published - Dec 2018 |
Abstract
In agricultural ecosystems, insect pests, pathogens, weather patterns, and reduced soil fertility pose major challenges to crop productivity and are responsible for significant yield losses worldwide. Iris yellow spot virus (IYSV) vectored by Thrips tabaci Lindeman, is a major hindrance to onion production in eastern Africa. Control measures often rely on insecticides with deleterious effects. Endophytes are one key alternative as they can play important roles in mediating induced systemic resistance. Hence, we examined the potential effect of endophytic fungus Hypocrea lixii (F3ST1) on feeding and replication of IYSV on endophyte-colonized (E+) and endophyte-free (E-) onion plants. For more precise assessment, replication was also tested using leaf disk bioassays and individual thrips. The number of feeding punctures was significantly lower in E+ as compared to E- plants. Disease level was significantly lower in E+ as compared to E- plants for four weeks post-exposure to thrips. IYSV replication was reduced by 2.5-fold in endophytic treatment on both whole plant and leaf disk assays. Thrips tabaci showed 2 times higher feeding activities on endophyte-free onion leaf disks as compared to the endophyte-inoculated leaf disks. Our results suggest potential utility of the endophytes to reduce feeding damage and virus infection on onion plants. Further studies should be conducted to elucidate the secondary metabolites involved in such endophyte-thrips-virus mediated interaction and determine whether the interactions extend for this and other onion varieties and viruses under field conditions.
Keywords
- Host plant resistance, Hypocrea lixii, Iris yellow spot virus, Multi-trophic interactions, Onions, Systemic, Thrips tabaci
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 9, 1785, 12.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Endophytic Colonization of Onions Induces Resistance Against Viruliferous Thrips and Virus Replication
AU - Muvea, Alexander Mutua
AU - Subramanian, Sevgan
AU - Maniania, Nguya Kalemba
AU - Poehling, Hans Michael
AU - Ekesi, Sunday
AU - Meyhöfer, Rainer
N1 - Funding information: The authors kindly acknowledge the receipt of polyclonal anti-NSs antibodies for IYSV from Dr. Hanu Pappu, Washington State University, Pullman. The authors thank Regina Malit for technical assistance during ELISA experiments and Peris Kariuki for screenhouse help. Funding. This study was funded by BMZ (The German Federal Ministry for Economic Cooperation and Development) through GIZ (Deutsche Gesellschaft für Internationale Zusammenarbeit) through a project grant entitled “Implementation of integrated thrips and tospovirus management strategies in small-holder vegetable cropping systems of Eastern Africa” (Project No. 11.7860.7-001.00, Contract No. 81141840) for which we are grateful. Core funding provided to icipe by United Kingdom Aid from the United Kingdom Government, Swedish International Development Cooperation Agency (Sida), the Swiss Agency for Development and Cooperation (SDC), Federal Ministry for Economic Cooperation and Development (BMZ), and Germany and the Kenyan Government. The publication of this article was funded by the Open Access fund of Leibniz Universität Hannover.
PY - 2018/12
Y1 - 2018/12
N2 - In agricultural ecosystems, insect pests, pathogens, weather patterns, and reduced soil fertility pose major challenges to crop productivity and are responsible for significant yield losses worldwide. Iris yellow spot virus (IYSV) vectored by Thrips tabaci Lindeman, is a major hindrance to onion production in eastern Africa. Control measures often rely on insecticides with deleterious effects. Endophytes are one key alternative as they can play important roles in mediating induced systemic resistance. Hence, we examined the potential effect of endophytic fungus Hypocrea lixii (F3ST1) on feeding and replication of IYSV on endophyte-colonized (E+) and endophyte-free (E-) onion plants. For more precise assessment, replication was also tested using leaf disk bioassays and individual thrips. The number of feeding punctures was significantly lower in E+ as compared to E- plants. Disease level was significantly lower in E+ as compared to E- plants for four weeks post-exposure to thrips. IYSV replication was reduced by 2.5-fold in endophytic treatment on both whole plant and leaf disk assays. Thrips tabaci showed 2 times higher feeding activities on endophyte-free onion leaf disks as compared to the endophyte-inoculated leaf disks. Our results suggest potential utility of the endophytes to reduce feeding damage and virus infection on onion plants. Further studies should be conducted to elucidate the secondary metabolites involved in such endophyte-thrips-virus mediated interaction and determine whether the interactions extend for this and other onion varieties and viruses under field conditions.
AB - In agricultural ecosystems, insect pests, pathogens, weather patterns, and reduced soil fertility pose major challenges to crop productivity and are responsible for significant yield losses worldwide. Iris yellow spot virus (IYSV) vectored by Thrips tabaci Lindeman, is a major hindrance to onion production in eastern Africa. Control measures often rely on insecticides with deleterious effects. Endophytes are one key alternative as they can play important roles in mediating induced systemic resistance. Hence, we examined the potential effect of endophytic fungus Hypocrea lixii (F3ST1) on feeding and replication of IYSV on endophyte-colonized (E+) and endophyte-free (E-) onion plants. For more precise assessment, replication was also tested using leaf disk bioassays and individual thrips. The number of feeding punctures was significantly lower in E+ as compared to E- plants. Disease level was significantly lower in E+ as compared to E- plants for four weeks post-exposure to thrips. IYSV replication was reduced by 2.5-fold in endophytic treatment on both whole plant and leaf disk assays. Thrips tabaci showed 2 times higher feeding activities on endophyte-free onion leaf disks as compared to the endophyte-inoculated leaf disks. Our results suggest potential utility of the endophytes to reduce feeding damage and virus infection on onion plants. Further studies should be conducted to elucidate the secondary metabolites involved in such endophyte-thrips-virus mediated interaction and determine whether the interactions extend for this and other onion varieties and viruses under field conditions.
KW - Host plant resistance
KW - Hypocrea lixii
KW - Iris yellow spot virus
KW - Multi-trophic interactions
KW - Onions
KW - Systemic
KW - Thrips tabaci
UR - http://www.scopus.com/inward/record.url?scp=85058781842&partnerID=8YFLogxK
U2 - 10.3389/fpls.2018.01785
DO - 10.3389/fpls.2018.01785
M3 - Article
C2 - 30574155
AN - SCOPUS:85058781842
VL - 9
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1785
ER -