Details
| Original language | English |
|---|---|
| Article number | 2100326 |
| Journal | Macromolecular Chemistry and Physics |
| Volume | 223 |
| Issue number | 2 |
| Early online date | 25 Nov 2021 |
| Publication status | Published - 22 Jan 2022 |
Abstract
This work presents enabling technologies for the optimization of the manufacturing of GelMA-based hydrogels constructs with desired stiffness gradients. The manufacturing technique combines dynamic mixing for gradient generation and a passive micromixer for efficient hydrogel blending. A digital replica of the fabrication process is developed, integrating theoretical and computational models, as well as experimental data, in order to predict and control the stiffness profile obtained within the constructs. The workflow for the development of the in silico framework, based on rigorous verification, validation, and uncertainty quantification steps, is presented. The validation of the digital replica is based on reference settings of process variables, which result in constructs with an exponential stiffness profile. The developed in silico model has been employed for optimizing process variables in order to obtain a linear stiffness profile in the extruded construct without the need of expensive and time-consuming trial-and-error procedures. The developed digital replica is now a powerful tool for the creation of hydrogel gradient constructs for tissue engineering applications or for the screening of optimal 3D cell culture conditions.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Condensed Matter Physics
- Chemistry(all)
- Physical and Theoretical Chemistry
- Materials Science(all)
- Polymers and Plastics
- Chemistry(all)
- Organic Chemistry
- Materials Science(all)
- Materials Chemistry
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In: Macromolecular Chemistry and Physics, Vol. 223, No. 2, 2100326, 22.01.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Enabling Technologies for Obtaining Desired Stiffness Gradients in GelMA Hydrogels Constructs
AU - Sauty, Bastien
AU - Santesarti, Gianluca
AU - Fleischhammer, Tabea
AU - Lindner, Patrick
AU - Lavrentieva, Antonina
AU - Pepelanova, Iliyana
AU - Marino, Michele
N1 - Funding Information: M.M. acknowledges the Italian Ministry of Education, University and Research (MIUR) for funding in the “Programma per Giovani Ricercatori ‐ anno 2017 Rita Levi Montalcini”. A.L. and I.P. acknowledge the support by the German Research Foundation (DFG Project 398007461 488 3D Dual‐Gradient Systems for Functional Cell Screening) and by the SMART BIOTECS initiative, financially supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. The authors also express their gratitude to Dr. Janina Bahnemann and her group for providing them with the 3D printed micromixer.
PY - 2022/1/22
Y1 - 2022/1/22
N2 - This work presents enabling technologies for the optimization of the manufacturing of GelMA-based hydrogels constructs with desired stiffness gradients. The manufacturing technique combines dynamic mixing for gradient generation and a passive micromixer for efficient hydrogel blending. A digital replica of the fabrication process is developed, integrating theoretical and computational models, as well as experimental data, in order to predict and control the stiffness profile obtained within the constructs. The workflow for the development of the in silico framework, based on rigorous verification, validation, and uncertainty quantification steps, is presented. The validation of the digital replica is based on reference settings of process variables, which result in constructs with an exponential stiffness profile. The developed in silico model has been employed for optimizing process variables in order to obtain a linear stiffness profile in the extruded construct without the need of expensive and time-consuming trial-and-error procedures. The developed digital replica is now a powerful tool for the creation of hydrogel gradient constructs for tissue engineering applications or for the screening of optimal 3D cell culture conditions.
AB - This work presents enabling technologies for the optimization of the manufacturing of GelMA-based hydrogels constructs with desired stiffness gradients. The manufacturing technique combines dynamic mixing for gradient generation and a passive micromixer for efficient hydrogel blending. A digital replica of the fabrication process is developed, integrating theoretical and computational models, as well as experimental data, in order to predict and control the stiffness profile obtained within the constructs. The workflow for the development of the in silico framework, based on rigorous verification, validation, and uncertainty quantification steps, is presented. The validation of the digital replica is based on reference settings of process variables, which result in constructs with an exponential stiffness profile. The developed in silico model has been employed for optimizing process variables in order to obtain a linear stiffness profile in the extruded construct without the need of expensive and time-consuming trial-and-error procedures. The developed digital replica is now a powerful tool for the creation of hydrogel gradient constructs for tissue engineering applications or for the screening of optimal 3D cell culture conditions.
UR - http://www.scopus.com/inward/record.url?scp=85121438374&partnerID=8YFLogxK
U2 - 10.1002/macp.202100326
DO - 10.1002/macp.202100326
M3 - Article
AN - SCOPUS:85121438374
VL - 223
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1022-1352
IS - 2
M1 - 2100326
ER -