Details
| Original language | English |
|---|---|
| Pages (from-to) | 337-347 |
| Number of pages | 11 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 86 |
| Issue number | 3 |
| Publication status | Published - 20 Jul 2006 |
Abstract
A protocol for plant regeneration from protoplasts has been developed, and then successfully applied to different genotypes of Cyclamen persicum Mill. Protoplasts were isolated from embryogenic suspension cultures by enzymatic digestion in 2% cellulase R10 and 0.5% macerozyme R10. Yields obtained varied between 1 and 5 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. The highest cell division frequencies were found in modified Kao and Michayluk (1975, Planta 126:105-110) medium containing the same types and concentrations of plant growth regulators that were applied for suspension culture (2.0 mg l-1 2,4-dichlorophenoxyacetic acid and 0.8 mg l-1 6-(γ,γ-dimethylallylamino)purine). Cell division was recorded for all 11 tested genotypes in frequencies of up to 12% and 18% after 7 and 14 days, respectively. However, cell division frequency varied strongly between different genotypes. After 4-6 weeks calluses were released from the alginate films and further cultured on hormone-containing medium for continued growth or transferred to hormone-free medium for regeneration of somatic embryos. Plant regeneration via somatic embryogenesis succeeded in 9 out of the 11 genotypes under investigation. Up to now protoplast-derived plants from four genotypes have been successfully transferred to soil.
Keywords
- Genotypic differences, Ornamental plant, Primulaceae, Protoplast culture, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 86, No. 3, 20.07.2006, p. 337-347.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Efficient plant regeneration from protoplasts isolated from embryogenic suspension cultures of Cyclamen persicum Mill.
AU - Winkelmann, Traud
AU - Specht, Janine
AU - Serek, Margrethe
PY - 2006/7/20
Y1 - 2006/7/20
N2 - A protocol for plant regeneration from protoplasts has been developed, and then successfully applied to different genotypes of Cyclamen persicum Mill. Protoplasts were isolated from embryogenic suspension cultures by enzymatic digestion in 2% cellulase R10 and 0.5% macerozyme R10. Yields obtained varied between 1 and 5 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. The highest cell division frequencies were found in modified Kao and Michayluk (1975, Planta 126:105-110) medium containing the same types and concentrations of plant growth regulators that were applied for suspension culture (2.0 mg l-1 2,4-dichlorophenoxyacetic acid and 0.8 mg l-1 6-(γ,γ-dimethylallylamino)purine). Cell division was recorded for all 11 tested genotypes in frequencies of up to 12% and 18% after 7 and 14 days, respectively. However, cell division frequency varied strongly between different genotypes. After 4-6 weeks calluses were released from the alginate films and further cultured on hormone-containing medium for continued growth or transferred to hormone-free medium for regeneration of somatic embryos. Plant regeneration via somatic embryogenesis succeeded in 9 out of the 11 genotypes under investigation. Up to now protoplast-derived plants from four genotypes have been successfully transferred to soil.
AB - A protocol for plant regeneration from protoplasts has been developed, and then successfully applied to different genotypes of Cyclamen persicum Mill. Protoplasts were isolated from embryogenic suspension cultures by enzymatic digestion in 2% cellulase R10 and 0.5% macerozyme R10. Yields obtained varied between 1 and 5 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. The highest cell division frequencies were found in modified Kao and Michayluk (1975, Planta 126:105-110) medium containing the same types and concentrations of plant growth regulators that were applied for suspension culture (2.0 mg l-1 2,4-dichlorophenoxyacetic acid and 0.8 mg l-1 6-(γ,γ-dimethylallylamino)purine). Cell division was recorded for all 11 tested genotypes in frequencies of up to 12% and 18% after 7 and 14 days, respectively. However, cell division frequency varied strongly between different genotypes. After 4-6 weeks calluses were released from the alginate films and further cultured on hormone-containing medium for continued growth or transferred to hormone-free medium for regeneration of somatic embryos. Plant regeneration via somatic embryogenesis succeeded in 9 out of the 11 genotypes under investigation. Up to now protoplast-derived plants from four genotypes have been successfully transferred to soil.
KW - Genotypic differences
KW - Ornamental plant
KW - Primulaceae
KW - Protoplast culture
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=33748714979&partnerID=8YFLogxK
U2 - 10.1007/s11240-006-9129-8
DO - 10.1007/s11240-006-9129-8
M3 - Article
AN - SCOPUS:33748714979
VL - 86
SP - 337
EP - 347
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -