Details
| Original language | English |
|---|---|
| Pages (from-to) | 161-165 |
| Number of pages | 5 |
| Journal | Food chemistry |
| Volume | 63 |
| Issue number | 2 |
| Publication status | Published - 30 Oct 1998 |
Abstract
2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Agricultural and Biological Sciences(all)
- Food Science
Sustainable Development Goals
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In: Food chemistry, Vol. 63, No. 2, 30.10.1998, p. 161-165.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Efficient generation of 2E-hexenal by a hydroperoxide lyase from mung bean seedlings
AU - Rehbock, B.
AU - Ganßer, D.
AU - Berger, R. G.
N1 - Funding information: This work was supported by the EU project AIR3-CT94-2060a nd by the Fonds der ChemischenIn dustrie, Frankfurt.
PY - 1998/10/30
Y1 - 1998/10/30
N2 - 2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.
AB - 2E-hexenal was generated with a high molar conversion rate by the incubation of a hydroperoxide lyase containing extract from mung bean seedlings and its substrate, 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT). Various parameters affected the yield of 2E-hexenal and the conversion rate: hydroperoxide lyase activity was especially, pronounced in seedlings older than 10 days. The cleavage of 13-HPOT by a solubilized enzyme extract proceeded best at pH 6.5. Time-course studies showed that the majority of flavour compounds was already generated during the first 10 min of the incubation period. Using optimized reaction conditions, a maximum yield of 1062 mg kg-1 2E-hexenal was obtained with 32.6 mmol kg-1 (10.1 gkg-1) 13-HPOT. However, the conversion rate was highest (maximum 86.3%) at a low substrate concentration, indicating a suicidal behaviour of the hydroperoxide lyase. Enzymatically hydrolyzed and dioxygenated linseed oil was a suitable alternative to pure linolenic acid.
UR - http://www.scopus.com/inward/record.url?scp=0032189743&partnerID=8YFLogxK
U2 - 10.1016/S0308-8146(98)00042-9
DO - 10.1016/S0308-8146(98)00042-9
M3 - Article
AN - SCOPUS:0032189743
VL - 63
SP - 161
EP - 165
JO - Food chemistry
JF - Food chemistry
SN - 0308-8146
IS - 2
ER -