Details
| Original language | English |
|---|---|
| Pages (from-to) | 171-182 |
| Number of pages | 12 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 101 |
| Issue number | 2 |
| Publication status | Published - 4 Feb 2010 |
Abstract
Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 × 105 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.
Keywords
- Flow cytometry, Ornamental plant, Ploidy stability, Protoplast isolation, Somaclonal variation, Vegetative propagation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 101, No. 2, 04.02.2010, p. 171-182.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Efficient and stable regeneration from protoplasts of Cyclamen coum Miller via somatic embryogenesis
AU - Prange, Anika Nadja Sabine
AU - Serek, Margrethe
AU - Bartsch, Melanie
AU - Winkelmann, Traud
N1 - Funding Information: Acknowledgments The authors thank Frank Schaarschmidt for his excellent guidance in the statistical data analysis. The financial support of the German Federal Ministry of Economics and Technology through the program PRO INNO grant no. KF0054802MD5 is gratefully acknowledged.
PY - 2010/2/4
Y1 - 2010/2/4
N2 - Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 × 105 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.
AB - Embryogenic cultures of Cyclamen coum were established on solid media and in suspension, and their growth characteristics in response to different concentrations of plant growth regulators (PGRs) were evaluated. Embryogenic cultures exhibited a high regeneration capacity of 876 somatic embryos per gram fresh mass. Up to 4.24 × 105 protoplasts per gram of fresh mass were isolated from somatic embryos and embryogenic suspension cultures. Protoplasts derived from both embryos and suspension cultures were successfully cultured in vitro and regenerated into plants via somatic embryogenesis. Phenotypic analyses and flow cytometric measurements revealed that some regenerated plants were tetraploid. About 20% of the protoplast-derived calluses used for regeneration were tetraploid, while tetraploidy was found in 0.9% of the plants regenerated from the embryogenic cultures.
KW - Flow cytometry
KW - Ornamental plant
KW - Ploidy stability
KW - Protoplast isolation
KW - Somaclonal variation
KW - Vegetative propagation
UR - http://www.scopus.com/inward/record.url?scp=77952009111&partnerID=8YFLogxK
U2 - 10.1007/s11240-010-9674-z
DO - 10.1007/s11240-010-9674-z
M3 - Article
AN - SCOPUS:77952009111
VL - 101
SP - 171
EP - 182
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 2
ER -