Details
| Original language | English |
|---|---|
| Pages (from-to) | 163-168 |
| Number of pages | 6 |
| Journal | Journal of biotechnology |
| Volume | 69 |
| Issue number | 2-3 |
| Publication status | Published - 15 Apr 1999 |
Abstract
The biotransformation of limonene by the basidiomycete Pleurotus sapidus yielded cis/trans-carveol and carvone as the main products. The transformation period was extended from 4 days after direct addition to 12 days by gas phase addition of the substrate. After 2 days of transformation, 97% of the substrate had accumulated in the mycelium, while only 3% were present in the culture medium. Substrate toxicity led to a decrease of dry matter. Adaptation of the precultures with small amounts of substrate doubled the concentration of carveol and increased the concentration of carvone by a factor of 3-4. Total product concentrations of >100 mg l-1 were reached. Copyright (C) 1999 Elsevier Science B.V.
Keywords
- Adaptation, Basidiomycete, Biotransformation, Induction, Limonene, Pleurotus sapidus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 69, No. 2-3, 15.04.1999, p. 163-168.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effects of R-(+)-limonene on submerged cultures of the terpene transforming basidiomycete Pleurotus sapidus
AU - Onken, J.
AU - Berger, R. G.
N1 - Funding information: This work was supported by the EU Project BIO4-CT95-0049 and by the Fonds der Chemischen Industrie, Frankfurt.
PY - 1999/4/15
Y1 - 1999/4/15
N2 - The biotransformation of limonene by the basidiomycete Pleurotus sapidus yielded cis/trans-carveol and carvone as the main products. The transformation period was extended from 4 days after direct addition to 12 days by gas phase addition of the substrate. After 2 days of transformation, 97% of the substrate had accumulated in the mycelium, while only 3% were present in the culture medium. Substrate toxicity led to a decrease of dry matter. Adaptation of the precultures with small amounts of substrate doubled the concentration of carveol and increased the concentration of carvone by a factor of 3-4. Total product concentrations of >100 mg l-1 were reached. Copyright (C) 1999 Elsevier Science B.V.
AB - The biotransformation of limonene by the basidiomycete Pleurotus sapidus yielded cis/trans-carveol and carvone as the main products. The transformation period was extended from 4 days after direct addition to 12 days by gas phase addition of the substrate. After 2 days of transformation, 97% of the substrate had accumulated in the mycelium, while only 3% were present in the culture medium. Substrate toxicity led to a decrease of dry matter. Adaptation of the precultures with small amounts of substrate doubled the concentration of carveol and increased the concentration of carvone by a factor of 3-4. Total product concentrations of >100 mg l-1 were reached. Copyright (C) 1999 Elsevier Science B.V.
KW - Adaptation
KW - Basidiomycete
KW - Biotransformation
KW - Induction
KW - Limonene
KW - Pleurotus sapidus
UR - http://www.scopus.com/inward/record.url?scp=0032965706&partnerID=8YFLogxK
U2 - 10.1016/S0168-1656(99)00040-1
DO - 10.1016/S0168-1656(99)00040-1
M3 - Article
C2 - 10361724
AN - SCOPUS:0032965706
VL - 69
SP - 163
EP - 168
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2-3
ER -