Details
| Original language | English |
|---|---|
| Pages (from-to) | 25752-25766 |
| Number of pages | 15 |
| Journal | Optics express |
| Volume | 24 |
| Issue number | 22 |
| Publication status | Published - Oct 2016 |
| Externally published | Yes |
Abstract
An inhomogeneous linear refractive index profile, such as that occurring in biological tissues, is shown to significantly alter stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy images. Our finite-difference time-domain simulations show that near-field enhancement and microlensing can lead to an increase of an object's perceived molecular density by a factor of nine and changes in its perceived position by 0.4 μm up to 1.0 μm. Thus the assumption that SRS scales linearly and CARS quadratically with density does not always hold. Furthermore, the inhomogeneous linear index can cause false CARS and AM-SRS signals, even for a homogeneous nonlinear susceptibility.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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In: Optics express, Vol. 24, No. 22, 10.2016, p. 25752-25766.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effects of refractive index mismatch on SRS and CARS microscopy
AU - Van Der Kolk, Jarno
AU - Lesina, Antonino Calà
AU - Ramunno, Lora
N1 - Funding information: Natural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs (CRC); Southern Ontario Smart Computing Innovation Platform (SOSCIP); the Canadian Foundation for Innovation (CFI); Ministry of Research and Innovation Ontario. We would like to thank Sharcnet for the computational resources that made this article possible (NRAP1007/NRAP1352, up to 256 cores per simulation on orca).
PY - 2016/10
Y1 - 2016/10
N2 - An inhomogeneous linear refractive index profile, such as that occurring in biological tissues, is shown to significantly alter stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy images. Our finite-difference time-domain simulations show that near-field enhancement and microlensing can lead to an increase of an object's perceived molecular density by a factor of nine and changes in its perceived position by 0.4 μm up to 1.0 μm. Thus the assumption that SRS scales linearly and CARS quadratically with density does not always hold. Furthermore, the inhomogeneous linear index can cause false CARS and AM-SRS signals, even for a homogeneous nonlinear susceptibility.
AB - An inhomogeneous linear refractive index profile, such as that occurring in biological tissues, is shown to significantly alter stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy images. Our finite-difference time-domain simulations show that near-field enhancement and microlensing can lead to an increase of an object's perceived molecular density by a factor of nine and changes in its perceived position by 0.4 μm up to 1.0 μm. Thus the assumption that SRS scales linearly and CARS quadratically with density does not always hold. Furthermore, the inhomogeneous linear index can cause false CARS and AM-SRS signals, even for a homogeneous nonlinear susceptibility.
UR - http://www.scopus.com/inward/record.url?scp=84995899634&partnerID=8YFLogxK
U2 - 10.1364/OE.24.025752
DO - 10.1364/OE.24.025752
M3 - Article
AN - SCOPUS:84995899634
VL - 24
SP - 25752
EP - 25766
JO - Optics express
JF - Optics express
SN - 1094-4087
IS - 22
ER -