Details
| Original language | English |
|---|---|
| Title of host publication | 6th International Symposium on Production and Establishment of Micropropagated Plants |
| Editors | E.A. Ozudogru, M. Beruto |
| Publisher | International Society for Horticultural Science |
| Pages | 337-341 |
| Number of pages | 5 |
| ISBN (electronic) | 9789462611 |
| Publication status | Published - 31 Mar 2017 |
| Event | VI International Symposium on Production and Establishment of Micropropagated Plants - San Remo, Italy Duration: 19 Apr 2015 → 24 Apr 2015 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 1155 |
| ISSN (Print) | 0567-7572 |
Abstract
Several species of Campanula genus have been cultivated as ornamental plants and there is still a growing demand for more attractive characters that could be achieved through breeding, including genetic manipulation. While competence and condition of transformed tissue have a direct effect on the success of gene transformation, obtaining an efficient protocol with balanced hormonal content, which induce healthy shoot regeneration, is necessary. To determine the optimum type and concentration of cytokinins, that induce shoot regeneration in Campanula portenschlagiana 'Blue Ocean', an experiment in two phases (I and II) was designed. In the initial phase (I), nodal cuts were harvested from mature greenhouse-grown plants and cultured on Murashige and Skoog (MS) media containing various concentrations of benzyladenine (BA) or thidiazuron (TDZ). In the second phase (II), petioles were harvested from the newly grown shoots, derived from the nodal cuts and were cultivated on MS media supplemented with different concentrations of BA or TDZ for shoot regeneration. Results revealed that the best rate of axillary node growth were exhibited by the nodal cuts, which were cultivated in medium containing 0.5 mg L-1 TDZ (in phase I). The most favorable shoot regeneration rate was obtained from petioles cultivated in 1.0 mg L-1 TDZ (in phase II). According to the results of this experiment, effect of hormone treatment in phase I on improving the shoot regeneration efficiency in phase II was not significant. Consequently, the final hormonal content of the media elicited a direct influence on the rate of shoot regeneration.
Keywords
- BA, Campanula, Cytokinins, Shoot regeneration, TDZ
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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6th International Symposium on Production and Establishment of Micropropagated Plants. ed. / E.A. Ozudogru; M. Beruto. International Society for Horticultural Science, 2017. p. 337-341 (Acta Horticulturae; Vol. 1155).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Effects of cytokinin treatment of nodal cuts and petioles on shoot regeneration of Campanula portenschlagiana 'Blue Ocean'
AU - Ghayoor Karimiani, Z.
AU - Mibus-Schoppe, Heiko
AU - Serek, Margrethe
N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/3/31
Y1 - 2017/3/31
N2 - Several species of Campanula genus have been cultivated as ornamental plants and there is still a growing demand for more attractive characters that could be achieved through breeding, including genetic manipulation. While competence and condition of transformed tissue have a direct effect on the success of gene transformation, obtaining an efficient protocol with balanced hormonal content, which induce healthy shoot regeneration, is necessary. To determine the optimum type and concentration of cytokinins, that induce shoot regeneration in Campanula portenschlagiana 'Blue Ocean', an experiment in two phases (I and II) was designed. In the initial phase (I), nodal cuts were harvested from mature greenhouse-grown plants and cultured on Murashige and Skoog (MS) media containing various concentrations of benzyladenine (BA) or thidiazuron (TDZ). In the second phase (II), petioles were harvested from the newly grown shoots, derived from the nodal cuts and were cultivated on MS media supplemented with different concentrations of BA or TDZ for shoot regeneration. Results revealed that the best rate of axillary node growth were exhibited by the nodal cuts, which were cultivated in medium containing 0.5 mg L-1 TDZ (in phase I). The most favorable shoot regeneration rate was obtained from petioles cultivated in 1.0 mg L-1 TDZ (in phase II). According to the results of this experiment, effect of hormone treatment in phase I on improving the shoot regeneration efficiency in phase II was not significant. Consequently, the final hormonal content of the media elicited a direct influence on the rate of shoot regeneration.
AB - Several species of Campanula genus have been cultivated as ornamental plants and there is still a growing demand for more attractive characters that could be achieved through breeding, including genetic manipulation. While competence and condition of transformed tissue have a direct effect on the success of gene transformation, obtaining an efficient protocol with balanced hormonal content, which induce healthy shoot regeneration, is necessary. To determine the optimum type and concentration of cytokinins, that induce shoot regeneration in Campanula portenschlagiana 'Blue Ocean', an experiment in two phases (I and II) was designed. In the initial phase (I), nodal cuts were harvested from mature greenhouse-grown plants and cultured on Murashige and Skoog (MS) media containing various concentrations of benzyladenine (BA) or thidiazuron (TDZ). In the second phase (II), petioles were harvested from the newly grown shoots, derived from the nodal cuts and were cultivated on MS media supplemented with different concentrations of BA or TDZ for shoot regeneration. Results revealed that the best rate of axillary node growth were exhibited by the nodal cuts, which were cultivated in medium containing 0.5 mg L-1 TDZ (in phase I). The most favorable shoot regeneration rate was obtained from petioles cultivated in 1.0 mg L-1 TDZ (in phase II). According to the results of this experiment, effect of hormone treatment in phase I on improving the shoot regeneration efficiency in phase II was not significant. Consequently, the final hormonal content of the media elicited a direct influence on the rate of shoot regeneration.
KW - BA
KW - Campanula
KW - Cytokinins
KW - Shoot regeneration
KW - TDZ
UR - http://www.scopus.com/inward/record.url?scp=85018764297&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2017.1155.49
DO - 10.17660/ActaHortic.2017.1155.49
M3 - Conference contribution
AN - SCOPUS:85018764297
T3 - Acta Horticulturae
SP - 337
EP - 341
BT - 6th International Symposium on Production and Establishment of Micropropagated Plants
A2 - Ozudogru, E.A.
A2 - Beruto, M.
PB - International Society for Horticultural Science
T2 - VI International Symposium on Production and Establishment of Micropropagated Plants
Y2 - 19 April 2015 through 24 April 2015
ER -