Details
| Original language | English |
|---|---|
| Pages (from-to) | 389-408 |
| Number of pages | 20 |
| Journal | Plant and soil |
| Volume | 406 |
| Issue number | 1-2 |
| Early online date | 15 Apr 2016 |
| Publication status | Published - Sept 2016 |
Abstract
Aims: The effects of biofumigation with Brassica juncea ‘Terra Plus’ and Raphanus sativus ‘Defender’ in comparison to Basamid on apple plant growth and on soil microbial communities were studied at three sites affected by replant disease under field conditions. Methods: Apple rootstocks were planted on differently treated plots to evaluate the effect of the treatments on plant growth under field and greenhouse conditions. The glucosinolates in biofumigant plant organs and their breakdown products in soils were determined. Denaturing gradient gel electrophoresis fingerprints were performed with 16S rRNA gene and ITS fragments amplified from total community DNA extracted from different soils. Results: The highest glucosinolate concentrations were found in inflorescences of both biofumigant plant species with no differences between sites. The most abundant degradation product in soil biofumigated with B. juncea was 2-propenyl isothiocyanate, while in soil treated with R. sativus only 4-(methylthio)-3-butenyl isothiocyanate was detected. Effects of biofumigation were recorded to be stronger on fungi than on bacteria. Growth of apple rootstocks was positively affected by the treatments in a site-dependent manner. Conclusions: The effects of biofumigation evaluated by the apple plant growth were site-dependent and might result from suppression of soil-borne pests and pathogens, changes in soil microbial community compositions, and additional nutrients from the incorporated biomass.
Keywords
- Apple replant disease, Bacterial community composition, Biofumigation, DGGE, Fungal community composition, Glucosinolate, Indicator plant, Isothiocyanate, Malus domestica
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Soil Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant and soil, Vol. 406, No. 1-2, 09.2016, p. 389-408.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effects of biofumigation using Brassica juncea and Raphanus sativus in comparison to disinfection using Basamid on apple plant growth and soil microbial communities at three field sites with replant disease
AU - Yim, B.
AU - Hanschen, F.S.
AU - Wrede, A.
AU - Nitt, H.
AU - Schreiner, M.
AU - Smalla, K.
AU - Winkelmann, T.
N1 - Funding Information: This work was supported by the German Federal Ministry of Food and Agriculture within the initiative “Bundesprogramm ökologischer Landbau und andere Formen nachhaltiger Landwirtschaft” (BÖLN). We thank the three nurseries K, A, and M, in Schleswig-Holstein for the project cooperation. We are also thankful to Stefan Weiß, Simon Richartz, Bärbel Ernst, Ewa Schneider, and Friederike Schröder for assistance in the biotest experiment, Andrea Jankowsky for help in GS determination, and Annett Platalla and Elke Büsch for determination of GS breakdown products. We also acknowledge Ilse-Marie Jungkurth, Philipp Braun, and Stefan Weiß for critically reading the manuscript.
PY - 2016/9
Y1 - 2016/9
N2 - Aims: The effects of biofumigation with Brassica juncea ‘Terra Plus’ and Raphanus sativus ‘Defender’ in comparison to Basamid on apple plant growth and on soil microbial communities were studied at three sites affected by replant disease under field conditions. Methods: Apple rootstocks were planted on differently treated plots to evaluate the effect of the treatments on plant growth under field and greenhouse conditions. The glucosinolates in biofumigant plant organs and their breakdown products in soils were determined. Denaturing gradient gel electrophoresis fingerprints were performed with 16S rRNA gene and ITS fragments amplified from total community DNA extracted from different soils. Results: The highest glucosinolate concentrations were found in inflorescences of both biofumigant plant species with no differences between sites. The most abundant degradation product in soil biofumigated with B. juncea was 2-propenyl isothiocyanate, while in soil treated with R. sativus only 4-(methylthio)-3-butenyl isothiocyanate was detected. Effects of biofumigation were recorded to be stronger on fungi than on bacteria. Growth of apple rootstocks was positively affected by the treatments in a site-dependent manner. Conclusions: The effects of biofumigation evaluated by the apple plant growth were site-dependent and might result from suppression of soil-borne pests and pathogens, changes in soil microbial community compositions, and additional nutrients from the incorporated biomass.
AB - Aims: The effects of biofumigation with Brassica juncea ‘Terra Plus’ and Raphanus sativus ‘Defender’ in comparison to Basamid on apple plant growth and on soil microbial communities were studied at three sites affected by replant disease under field conditions. Methods: Apple rootstocks were planted on differently treated plots to evaluate the effect of the treatments on plant growth under field and greenhouse conditions. The glucosinolates in biofumigant plant organs and their breakdown products in soils were determined. Denaturing gradient gel electrophoresis fingerprints were performed with 16S rRNA gene and ITS fragments amplified from total community DNA extracted from different soils. Results: The highest glucosinolate concentrations were found in inflorescences of both biofumigant plant species with no differences between sites. The most abundant degradation product in soil biofumigated with B. juncea was 2-propenyl isothiocyanate, while in soil treated with R. sativus only 4-(methylthio)-3-butenyl isothiocyanate was detected. Effects of biofumigation were recorded to be stronger on fungi than on bacteria. Growth of apple rootstocks was positively affected by the treatments in a site-dependent manner. Conclusions: The effects of biofumigation evaluated by the apple plant growth were site-dependent and might result from suppression of soil-borne pests and pathogens, changes in soil microbial community compositions, and additional nutrients from the incorporated biomass.
KW - Apple replant disease
KW - Bacterial community composition
KW - Biofumigation
KW - DGGE
KW - Fungal community composition
KW - Glucosinolate
KW - Indicator plant
KW - Isothiocyanate
KW - Malus domestica
UR - http://www.scopus.com/inward/record.url?scp=84963738065&partnerID=8YFLogxK
U2 - 10.1007/s11104-016-2876-3
DO - 10.1007/s11104-016-2876-3
M3 - Article
VL - 406
SP - 389
EP - 408
JO - Plant and soil
JF - Plant and soil
SN - 0032-079X
IS - 1-2
ER -