Details
| Original language | English |
|---|---|
| Pages (from-to) | 291-294 |
| Number of pages | 4 |
| Journal | Separation and Purification Technology |
| Volume | 49 |
| Issue number | 3 |
| Publication status | Published - 1 Dec 2005 |
Abstract
An increasing demand of lignin-degrading enzymes makes an effective enrichment of laccase C from culture broth necessary. In this work, the continuous foam fractionation of laccase C was investigated as an alternative to other common applied methods, such as salting-out, dialyses or ultrafiltration, which are usually accompanied by losses of catalytic activity or low operational capacity. After optimization of different process parameters (feed position, gas flow rate and surfactant concentration) an enrichment ratio of 11.7 was achieved for laccase C along with 70.2% recovery. Feed position at the top of the foaming column increased the recovery. Increasing the gas flow rate led to a lower enrichment but higher recovery, and an increased outlet flow rate had only negative effect on the recovery. With increasing concentration of cetyl trimethyl ammonium bromide (CTAB), which was applied as a surfactant to enable foaming, the enrichment ratio decreased (from 11.7 to 1.8) and recovery increased (from 70.2 up to 80.2%). A for the enrichment ratio and recovery optimum CTAB-concentration of 0.4 mg/ml was found. The method has been proven to be effective for the enrichment of laccase C.
Keywords
- Continuous foam fractionation, Enrichment ratio, Laccase C, Process parameters, Recovery
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Chemical Engineering(all)
- Filtration and Separation
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In: Separation and Purification Technology, Vol. 49, No. 3, 01.12.2005, p. 291-294.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effective enrichment and recovery of laccase C using continuous foam fractionation
AU - Gerken, Birte M.
AU - Nicolai, Andreas
AU - Linke, Diana
AU - Zorn, Holger
AU - Berger, Ralf G.
AU - Parlar, Harun
N1 - Funding information: Financial support by the German Federation of Industrial Research Associations “Otto von Guericke” (AiF) through the Research Association of the German Food Industry (FEI) is gratefully acknowledged (project # 121 ZN).
PY - 2005/12/1
Y1 - 2005/12/1
N2 - An increasing demand of lignin-degrading enzymes makes an effective enrichment of laccase C from culture broth necessary. In this work, the continuous foam fractionation of laccase C was investigated as an alternative to other common applied methods, such as salting-out, dialyses or ultrafiltration, which are usually accompanied by losses of catalytic activity or low operational capacity. After optimization of different process parameters (feed position, gas flow rate and surfactant concentration) an enrichment ratio of 11.7 was achieved for laccase C along with 70.2% recovery. Feed position at the top of the foaming column increased the recovery. Increasing the gas flow rate led to a lower enrichment but higher recovery, and an increased outlet flow rate had only negative effect on the recovery. With increasing concentration of cetyl trimethyl ammonium bromide (CTAB), which was applied as a surfactant to enable foaming, the enrichment ratio decreased (from 11.7 to 1.8) and recovery increased (from 70.2 up to 80.2%). A for the enrichment ratio and recovery optimum CTAB-concentration of 0.4 mg/ml was found. The method has been proven to be effective for the enrichment of laccase C.
AB - An increasing demand of lignin-degrading enzymes makes an effective enrichment of laccase C from culture broth necessary. In this work, the continuous foam fractionation of laccase C was investigated as an alternative to other common applied methods, such as salting-out, dialyses or ultrafiltration, which are usually accompanied by losses of catalytic activity or low operational capacity. After optimization of different process parameters (feed position, gas flow rate and surfactant concentration) an enrichment ratio of 11.7 was achieved for laccase C along with 70.2% recovery. Feed position at the top of the foaming column increased the recovery. Increasing the gas flow rate led to a lower enrichment but higher recovery, and an increased outlet flow rate had only negative effect on the recovery. With increasing concentration of cetyl trimethyl ammonium bromide (CTAB), which was applied as a surfactant to enable foaming, the enrichment ratio decreased (from 11.7 to 1.8) and recovery increased (from 70.2 up to 80.2%). A for the enrichment ratio and recovery optimum CTAB-concentration of 0.4 mg/ml was found. The method has been proven to be effective for the enrichment of laccase C.
KW - Continuous foam fractionation
KW - Enrichment ratio
KW - Laccase C
KW - Process parameters
KW - Recovery
UR - http://www.scopus.com/inward/record.url?scp=33646079417&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2005.09.015
DO - 10.1016/j.seppur.2005.09.015
M3 - Article
AN - SCOPUS:33646079417
VL - 49
SP - 291
EP - 294
JO - Separation and Purification Technology
JF - Separation and Purification Technology
SN - 1383-5866
IS - 3
ER -