Details
| Original language | English |
|---|---|
| Pages (from-to) | 210-214 |
| Number of pages | 5 |
| Journal | Soil Biology and Biochemistry |
| Volume | 125 |
| Early online date | 18 Jul 2018 |
| Publication status | Published - Oct 2018 |
Abstract
We investigated the response of aerobic methane oxidation and the associated methanotrophs to salt-stress in a NaCl gradient ranging from 0 M (un-amended reference) to 0.6 M NaCl (seawater salinity) using a rice paddy soil as a model system. Salt-stress significantly inhibited methanotrophic activity at > 0.3 M NaCl; at 0.6 M NaCl amendment, methanotrophic activity fully ceased. MiSeq sequencing of the pmoA gene and group-specific qPCR analyses revealed that type Ia methanotroph (Methylobacter) appeared to be favored under salinity up to 0.3 M NaCl, increasing in numerical abundance, while the type Ib was adversely affected. This suggests niche differentiation within members of the gammaproteobacterial methanotrophs. Overall, rice paddy soil methanotrophs showed remarkable resistance to salt-stress.
Keywords
- Global-change, NaCl-Amendment, Rice paddy, Salinization, pmoA
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
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In: Soil Biology and Biochemistry, Vol. 125, 10.2018, p. 210-214.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of salt stress on aerobic methane oxidation and associated methanotrophs; a microcosm study of a natural community from a non-saline environment
AU - Ho, Adrian
AU - Mo, Yongliang
AU - Lee, Hyo Jung
AU - Sauheitl, Leopold
AU - Jia, Zhongjun
AU - Horn, Marcus A.
N1 - Funding information: We are grateful to Natalie Röder for excellent technical assistance. Daria Frohloff and Stefanie Hetz are acknowledged for their help with the ammonium assay. AH is financially supported by the Leibniz Universität Hannover (Hannover, Germany). We are grateful for financial support by the grant DFG HO4020/3-1 . We are grateful to Natalie Röder for excellent technical assistance. Daria Frohloff and Stefanie Hetz are acknowledged for their help with the ammonium assay. AH is financially supported by the Leibniz Universität Hannover (Hannover, Germany). We are grateful for financial support by the grant DFG HO4020/3-1.
PY - 2018/10
Y1 - 2018/10
N2 - We investigated the response of aerobic methane oxidation and the associated methanotrophs to salt-stress in a NaCl gradient ranging from 0 M (un-amended reference) to 0.6 M NaCl (seawater salinity) using a rice paddy soil as a model system. Salt-stress significantly inhibited methanotrophic activity at > 0.3 M NaCl; at 0.6 M NaCl amendment, methanotrophic activity fully ceased. MiSeq sequencing of the pmoA gene and group-specific qPCR analyses revealed that type Ia methanotroph (Methylobacter) appeared to be favored under salinity up to 0.3 M NaCl, increasing in numerical abundance, while the type Ib was adversely affected. This suggests niche differentiation within members of the gammaproteobacterial methanotrophs. Overall, rice paddy soil methanotrophs showed remarkable resistance to salt-stress.
AB - We investigated the response of aerobic methane oxidation and the associated methanotrophs to salt-stress in a NaCl gradient ranging from 0 M (un-amended reference) to 0.6 M NaCl (seawater salinity) using a rice paddy soil as a model system. Salt-stress significantly inhibited methanotrophic activity at > 0.3 M NaCl; at 0.6 M NaCl amendment, methanotrophic activity fully ceased. MiSeq sequencing of the pmoA gene and group-specific qPCR analyses revealed that type Ia methanotroph (Methylobacter) appeared to be favored under salinity up to 0.3 M NaCl, increasing in numerical abundance, while the type Ib was adversely affected. This suggests niche differentiation within members of the gammaproteobacterial methanotrophs. Overall, rice paddy soil methanotrophs showed remarkable resistance to salt-stress.
KW - Global-change
KW - NaCl-Amendment
KW - Rice paddy
KW - Salinization
KW - pmoA
UR - http://www.scopus.com/inward/record.url?scp=85049937883&partnerID=8YFLogxK
U2 - 10.15488/15939
DO - 10.15488/15939
M3 - Article
VL - 125
SP - 210
EP - 214
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
ER -