Details
| Original language | English |
|---|---|
| Pages (from-to) | 5082-5085 |
| Number of pages | 4 |
| Journal | Optics letters |
| Volume | 43 |
| Issue number | 20 |
| Publication status | Published - 15 Oct 2018 |
| Externally published | Yes |
Abstract
Nonlinear optical imaging in the epi-direction is used to image subresolution features. We find that a refractive index mismatch between the object to be imaged and the background medium can change the far-field intensity image. As an example, we study second harmonic generation (SHG) microscopy where the forward-to-backward (F/B) ratio is used to quantify subresolution features. We show both theoretically and experimentally that the inhomogeneous refractive index in collagen tendon tissue creates near-field effects, which can change the F/B ratio by ∼20%-25%, even though the effect is negligible for most of the individual fibrils in the tissue. This is caused by the sensitivity of the backward signal on phase matching conditions.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
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In: Optics letters, Vol. 43, No. 20, 15.10.2018, p. 5082-5085.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of refractive index mismatch on forward-to-backward ratios in SHG imaging
AU - Van Der Kolk, Jarno N.
AU - Bancelin, Stéphane
AU - Kioulos, Charalambos
AU - Lesina, Antonino Calà
AU - Légaré, François
AU - Ramunno, Lora
N1 - Funding information: Canada Foundation for Innovation (CFI); Ontario Ministry of Research, Innovation and Science (MRIS); Natural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs; SOSCIP.
PY - 2018/10/15
Y1 - 2018/10/15
N2 - Nonlinear optical imaging in the epi-direction is used to image subresolution features. We find that a refractive index mismatch between the object to be imaged and the background medium can change the far-field intensity image. As an example, we study second harmonic generation (SHG) microscopy where the forward-to-backward (F/B) ratio is used to quantify subresolution features. We show both theoretically and experimentally that the inhomogeneous refractive index in collagen tendon tissue creates near-field effects, which can change the F/B ratio by ∼20%-25%, even though the effect is negligible for most of the individual fibrils in the tissue. This is caused by the sensitivity of the backward signal on phase matching conditions.
AB - Nonlinear optical imaging in the epi-direction is used to image subresolution features. We find that a refractive index mismatch between the object to be imaged and the background medium can change the far-field intensity image. As an example, we study second harmonic generation (SHG) microscopy where the forward-to-backward (F/B) ratio is used to quantify subresolution features. We show both theoretically and experimentally that the inhomogeneous refractive index in collagen tendon tissue creates near-field effects, which can change the F/B ratio by ∼20%-25%, even though the effect is negligible for most of the individual fibrils in the tissue. This is caused by the sensitivity of the backward signal on phase matching conditions.
UR - http://www.scopus.com/inward/record.url?scp=85054898679&partnerID=8YFLogxK
U2 - 10.1364/ol.43.005082
DO - 10.1364/ol.43.005082
M3 - Article
C2 - 30320824
AN - SCOPUS:85054898679
VL - 43
SP - 5082
EP - 5085
JO - Optics letters
JF - Optics letters
SN - 0146-9592
IS - 20
ER -