Details
| Original language | English |
|---|---|
| Article number | 4 |
| Number of pages | 9 |
| Journal | Lipids in health and disease |
| Volume | 14 |
| Issue number | 1 |
| Publication status | Published - 14 Feb 2015 |
Abstract
BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level.
METHODS: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging.
RESULTS: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation.
CONCLUSIONS: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion.
Keywords
- Actin-Related Protein 2-3 Complex/drug effects, Adult, Cofilin 1/drug effects, Cytoskeleton/drug effects, Down-Regulation/drug effects, Fatty Acids, Omega-3/pharmacology, Fluorescent Antibody Technique, Gene Expression/drug effects, Humans, Lim Kinases/drug effects, Male, Real-Time Polymerase Chain Reaction, Wiskott-Aldrich Syndrome Protein Family/drug effects, Wiskott-Aldrich Syndrome Protein, Neuronal/drug effects, cdc42 GTP-Binding Protein/drug effects, p21-Activated Kinases/drug effects, rac1 GTP-Binding Protein/drug effects, rho-Associated Kinases/drug effects, rhoA GTP-Binding Protein/drug effects, Omega-3 index, Red blood cells, Gene-expression, GTPases, n-3 PUFA, F-actin, Cytoskeleton
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, medical
- Biochemistry, Genetics and Molecular Biology(all)
- Endocrinology
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Medicine(all)
- Endocrinology, Diabetes and Metabolism
Sustainable Development Goals
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Lipids in health and disease, Vol. 14, No. 1, 4, 14.02.2015.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton
T2 - an open-label intervention study
AU - Schmidt, Simone
AU - Willers, Janina
AU - Riecker, Sabine
AU - Möller, Katharina
AU - Schuchardt, Jan Philipp
AU - Hahn, Andreas
N1 - Funding information: This study was supported by the young researcher program “Wege in die Forschung” of the Leibniz University of Hannover, Germany. We thank the Department of Cell Biology of the Medical School Hannover (MHH), which enabled us to use their confocal laser microscope for analysis. The supply of the study preparations from Dr. Loges + Co. GmbH, Winsen, Germany, is gratefully acknowledged.
PY - 2015/2/14
Y1 - 2015/2/14
N2 - BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level.METHODS: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging.RESULTS: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation.CONCLUSIONS: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion.
AB - BACKGROUND: Omega-3 polyunsaturated fatty acids (n-3 PUFAs) show beneficial effects on cardiovascular health and cognitive functions, but the underlying molecular mechanisms are not completely understood. Because of the fact that cytoskeleton dynamics affect almost every cellular process, the regulation of cytoskeletal dynamics could be a new pathway by which n-3 PUFAs exert their effects on cellular level.METHODS: A 12-week open-label intervention study with 12 healthy men was conducted to determine the effects of 2.7 g/d n-3 PUFA on changes in mRNA expression of cytoskeleton-associated genes by quantitative real-time PCR in whole blood. Furthermore, the actin content in red blood cells was analyzed by immunofluorescence imaging.RESULTS: N-3 PUFA supplementation resulted in a significant down-regulation of cytoskeleton-associated genes, in particular three GTPases (RAC1, RHOA, CDC42), three kinases (ROCK1, PAK2, LIMK), two Wiskott-Aldrich syndrome proteins (WASL, WASF2) as well as actin related protein 2/3 complex (ARPC2, ARPC3) and cofilin (CFL1). Variability in F-actin content between subjects was high; reduced actin content was only reduced within group evaluation.CONCLUSIONS: Reduced cytoskeleton-associated gene expression after n-3 PUFA supplementation suggests that regulation of cytoskeleton dynamics might be an additional way by which n-3 PUFAs exert their cellular effects. Concerning F-actin, this analysis did not reveal unmistakable results impeding a generalized conclusion.
KW - Actin-Related Protein 2-3 Complex/drug effects
KW - Adult
KW - Cofilin 1/drug effects
KW - Cytoskeleton/drug effects
KW - Down-Regulation/drug effects
KW - Fatty Acids, Omega-3/pharmacology
KW - Fluorescent Antibody Technique
KW - Gene Expression/drug effects
KW - Humans
KW - Lim Kinases/drug effects
KW - Male
KW - Real-Time Polymerase Chain Reaction
KW - Wiskott-Aldrich Syndrome Protein Family/drug effects
KW - Wiskott-Aldrich Syndrome Protein, Neuronal/drug effects
KW - cdc42 GTP-Binding Protein/drug effects
KW - p21-Activated Kinases/drug effects
KW - rac1 GTP-Binding Protein/drug effects
KW - rho-Associated Kinases/drug effects
KW - rhoA GTP-Binding Protein/drug effects
KW - Omega-3 index
KW - Red blood cells
KW - Gene-expression
KW - GTPases
KW - n-3 PUFA
KW - F-actin
KW - Cytoskeleton
UR - http://www.scopus.com/inward/record.url?scp=84929176846&partnerID=8YFLogxK
U2 - 10.1186/1476-511X-14-4
DO - 10.1186/1476-511X-14-4
M3 - Article
C2 - 25971815
VL - 14
JO - Lipids in health and disease
JF - Lipids in health and disease
SN - 1476-511X
IS - 1
M1 - 4
ER -