Details
| Original language | English |
|---|---|
| Pages (from-to) | 48-55 |
| Number of pages | 8 |
| Journal | International Biodeterioration and Biodegradation |
| Volume | 83 |
| Early online date | 10 May 2013 |
| Publication status | Published - Sept 2013 |
Abstract
The present study is focused on the effect of synthetic surfactants, at low concentration, on the kinetics of polycyclic aromatic hydrocarbons (PAH) biodegradation by Pseudomonas putida ATCC 17514 and addresses the specific issue of the effect of the surfactant on bacterial adhesion to PAH, which is believed to be an important mechanism for the uptake of hydrophobic compounds. For that purpose, three surfactants were tested, namely, the nonionic Tween 20, the anionic sodium dodecyl sulphate (SDS) and the cationic surfactant cetyltrymethyl ammonium bromide (CTAB). Data showed that the effect of each surfactant on the ability of strain ATCC 17514 to biodegrade fluoranthene and anthracene and to use them as growth substrate varied considerably. Tween 20, at a concentration of 0.08mM, increased the biodegradation rate of fluoranthene and doubled the maximum specific biodegradation rate of anthracene. The presence of SDS, at a concentration of 0.35mM, led to a reduction of 50% on the biodegradation rate of fluoranthene, but doubled the removal rate of the more hydrophobic anthracene (0.3mg L-1h-1). Finally, CTAB, at a concentration of 0.27mM, had a negative effect on the biodegradation of both PAH, leading to an abrupt decrease on the biomass growth.
Keywords
- Anthracene, Biodegradation, Fluoranthene, PAH, Pseudomonas, Surfactant
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Materials Science(all)
- Biomaterials
- Environmental Science(all)
- Waste Management and Disposal
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In: International Biodeterioration and Biodegradation, Vol. 83, 09.2013, p. 48-55.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Effect of low concentrations of synthetic surfactants on polycyclic aromatic hydrocarbons (PAH) biodegradation
AU - Rodrigues, Ana
AU - Nogueira, Regina
AU - Melo, Luís F.
AU - Brito, António G.
PY - 2013/9
Y1 - 2013/9
N2 - The present study is focused on the effect of synthetic surfactants, at low concentration, on the kinetics of polycyclic aromatic hydrocarbons (PAH) biodegradation by Pseudomonas putida ATCC 17514 and addresses the specific issue of the effect of the surfactant on bacterial adhesion to PAH, which is believed to be an important mechanism for the uptake of hydrophobic compounds. For that purpose, three surfactants were tested, namely, the nonionic Tween 20, the anionic sodium dodecyl sulphate (SDS) and the cationic surfactant cetyltrymethyl ammonium bromide (CTAB). Data showed that the effect of each surfactant on the ability of strain ATCC 17514 to biodegrade fluoranthene and anthracene and to use them as growth substrate varied considerably. Tween 20, at a concentration of 0.08mM, increased the biodegradation rate of fluoranthene and doubled the maximum specific biodegradation rate of anthracene. The presence of SDS, at a concentration of 0.35mM, led to a reduction of 50% on the biodegradation rate of fluoranthene, but doubled the removal rate of the more hydrophobic anthracene (0.3mg L-1h-1). Finally, CTAB, at a concentration of 0.27mM, had a negative effect on the biodegradation of both PAH, leading to an abrupt decrease on the biomass growth.
AB - The present study is focused on the effect of synthetic surfactants, at low concentration, on the kinetics of polycyclic aromatic hydrocarbons (PAH) biodegradation by Pseudomonas putida ATCC 17514 and addresses the specific issue of the effect of the surfactant on bacterial adhesion to PAH, which is believed to be an important mechanism for the uptake of hydrophobic compounds. For that purpose, three surfactants were tested, namely, the nonionic Tween 20, the anionic sodium dodecyl sulphate (SDS) and the cationic surfactant cetyltrymethyl ammonium bromide (CTAB). Data showed that the effect of each surfactant on the ability of strain ATCC 17514 to biodegrade fluoranthene and anthracene and to use them as growth substrate varied considerably. Tween 20, at a concentration of 0.08mM, increased the biodegradation rate of fluoranthene and doubled the maximum specific biodegradation rate of anthracene. The presence of SDS, at a concentration of 0.35mM, led to a reduction of 50% on the biodegradation rate of fluoranthene, but doubled the removal rate of the more hydrophobic anthracene (0.3mg L-1h-1). Finally, CTAB, at a concentration of 0.27mM, had a negative effect on the biodegradation of both PAH, leading to an abrupt decrease on the biomass growth.
KW - Anthracene
KW - Biodegradation
KW - Fluoranthene
KW - PAH
KW - Pseudomonas
KW - Surfactant
UR - http://www.scopus.com/inward/record.url?scp=84877834196&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2013.04.001
DO - 10.1016/j.ibiod.2013.04.001
M3 - Article
AN - SCOPUS:84877834196
VL - 83
SP - 48
EP - 55
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
SN - 0964-8305
ER -