Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Christian Senwitz
  • Daniel Butscher
  • Linus Holtmann
  • Manja Vogel
  • Robin Steudtner
  • Björn Drobot
  • Thorsten Stumpf
  • Astrid Barkleit
  • Anne Heller

External Research Organisations

  • Technische Universität Dresden
  • VKTA – Strahlenschutz, Analytik & Entsorgung Rossendorf e.V (VKTA)
  • Helmholtz-Zentrum Dresden-Rossendorf (HZDR)
View graph of relations

Details

Original languageEnglish
Article number171374
Number of pages20
JournalScience of the Total Environment
Volume923
Early online date2 Mar 2024
Publication statusPublished - 1 May 2024

Abstract

Heavy metals pose a potential health risk to humans when they enter the organism. Renal excretion is one of the elimination pathways and, therefore, investigations with kidney cells are of particular interest. In the present study, the effects of Ba(II), Eu(III), and U(VI) on rat and human renal cells were investigated in vitro. A combination of microscopic, biochemical, analytical, and spectroscopic methods was used to assess cell viability, cell death mechanisms, and intracellular metal uptake of exposed cells as well as metal speciation in cell culture medium and inside cells. For Eu(III) and U(VI), cytotoxicity and intracellular uptake are positively correlated and depend on concentration and exposure time. An enhanced apoptosis occurs upon Eu(III) exposure whereas U(VI) exposure leads to enhanced apoptosis and (secondary) necrosis. In contrast to that, Ba(II) exhibits no cytotoxic effect at all and its intracellular uptake is time-independently very low. In general, both cell lines give similar results with rat cells being more sensitive than human cells. The dominant binding motifs of Eu(III) in cell culture medium as well as cell suspensions are (organo-) phosphate groups. Additionally, a protein complex is formed in medium at low Eu(III) concentration. In contrast, U(VI) forms a carbonate complex in cell culture medium as well as each one phosphate and carbonate complex in cell suspensions. Using chemical microscopy, Eu(III) was localized in granular, vesicular compartments near the nucleus and the intracellular Eu(III) species equals the one in cell suspensions. Overall, this study contributes to a better understanding of the interactions of Ba(II), Eu(III), and U(VI) on a cellular and molecular level. Since Ba(II) and Eu(III) serve as inactive analogs of the radioactive Ra(II) and Am(III)/Cm(III), the results of this study are also of importance for the health risk assessment of these radionuclides.

Keywords

    Chemical microscopy, Cytotoxicity, Heavy metal speciation, Kidney cells, Radionuclides, TRLFS

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro. / Senwitz, Christian; Butscher, Daniel; Holtmann, Linus et al.
In: Science of the Total Environment, Vol. 923, 171374, 01.05.2024.

Research output: Contribution to journalArticleResearchpeer review

Senwitz, C, Butscher, D, Holtmann, L, Vogel, M, Steudtner, R, Drobot, B, Stumpf, T, Barkleit, A & Heller, A 2024, 'Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro', Science of the Total Environment, vol. 923, 171374. https://doi.org/10.1016/j.scitotenv.2024.171374
Senwitz, C., Butscher, D., Holtmann, L., Vogel, M., Steudtner, R., Drobot, B., Stumpf, T., Barkleit, A., & Heller, A. (2024). Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro. Science of the Total Environment, 923, Article 171374. https://doi.org/10.1016/j.scitotenv.2024.171374
Senwitz C, Butscher D, Holtmann L, Vogel M, Steudtner R, Drobot B et al. Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro. Science of the Total Environment. 2024 May 1;923:171374. Epub 2024 Mar 2. doi: 10.1016/j.scitotenv.2024.171374
Senwitz, Christian ; Butscher, Daniel ; Holtmann, Linus et al. / Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro. In: Science of the Total Environment. 2024 ; Vol. 923.
Download
@article{de2c2ec8dbb34eb68ade5a869df91e27,
title = "Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro",
abstract = "Heavy metals pose a potential health risk to humans when they enter the organism. Renal excretion is one of the elimination pathways and, therefore, investigations with kidney cells are of particular interest. In the present study, the effects of Ba(II), Eu(III), and U(VI) on rat and human renal cells were investigated in vitro. A combination of microscopic, biochemical, analytical, and spectroscopic methods was used to assess cell viability, cell death mechanisms, and intracellular metal uptake of exposed cells as well as metal speciation in cell culture medium and inside cells. For Eu(III) and U(VI), cytotoxicity and intracellular uptake are positively correlated and depend on concentration and exposure time. An enhanced apoptosis occurs upon Eu(III) exposure whereas U(VI) exposure leads to enhanced apoptosis and (secondary) necrosis. In contrast to that, Ba(II) exhibits no cytotoxic effect at all and its intracellular uptake is time-independently very low. In general, both cell lines give similar results with rat cells being more sensitive than human cells. The dominant binding motifs of Eu(III) in cell culture medium as well as cell suspensions are (organo-) phosphate groups. Additionally, a protein complex is formed in medium at low Eu(III) concentration. In contrast, U(VI) forms a carbonate complex in cell culture medium as well as each one phosphate and carbonate complex in cell suspensions. Using chemical microscopy, Eu(III) was localized in granular, vesicular compartments near the nucleus and the intracellular Eu(III) species equals the one in cell suspensions. Overall, this study contributes to a better understanding of the interactions of Ba(II), Eu(III), and U(VI) on a cellular and molecular level. Since Ba(II) and Eu(III) serve as inactive analogs of the radioactive Ra(II) and Am(III)/Cm(III), the results of this study are also of importance for the health risk assessment of these radionuclides.",
keywords = "Chemical microscopy, Cytotoxicity, Heavy metal speciation, Kidney cells, Radionuclides, TRLFS",
author = "Christian Senwitz and Daniel Butscher and Linus Holtmann and Manja Vogel and Robin Steudtner and Bj{\"o}rn Drobot and Thorsten Stumpf and Astrid Barkleit and Anne Heller",
note = "Funding Information: This work was funded by the German Federal Ministry of Education and Research (BMBF) under grant numbers 02NUK057A and 02NUK057B and is part of the joint project RADEKOR. L.H. acknowledges funding received from BMBF under grant number 02NUK057D ",
year = "2024",
month = may,
day = "1",
doi = "10.1016/j.scitotenv.2024.171374",
language = "English",
volume = "923",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

Download

TY - JOUR

T1 - Effect of Ba(II), Eu(III), and U(VI) on rat NRK-52E and human HEK-293 kidney cells in vitro

AU - Senwitz, Christian

AU - Butscher, Daniel

AU - Holtmann, Linus

AU - Vogel, Manja

AU - Steudtner, Robin

AU - Drobot, Björn

AU - Stumpf, Thorsten

AU - Barkleit, Astrid

AU - Heller, Anne

N1 - Funding Information: This work was funded by the German Federal Ministry of Education and Research (BMBF) under grant numbers 02NUK057A and 02NUK057B and is part of the joint project RADEKOR. L.H. acknowledges funding received from BMBF under grant number 02NUK057D

PY - 2024/5/1

Y1 - 2024/5/1

N2 - Heavy metals pose a potential health risk to humans when they enter the organism. Renal excretion is one of the elimination pathways and, therefore, investigations with kidney cells are of particular interest. In the present study, the effects of Ba(II), Eu(III), and U(VI) on rat and human renal cells were investigated in vitro. A combination of microscopic, biochemical, analytical, and spectroscopic methods was used to assess cell viability, cell death mechanisms, and intracellular metal uptake of exposed cells as well as metal speciation in cell culture medium and inside cells. For Eu(III) and U(VI), cytotoxicity and intracellular uptake are positively correlated and depend on concentration and exposure time. An enhanced apoptosis occurs upon Eu(III) exposure whereas U(VI) exposure leads to enhanced apoptosis and (secondary) necrosis. In contrast to that, Ba(II) exhibits no cytotoxic effect at all and its intracellular uptake is time-independently very low. In general, both cell lines give similar results with rat cells being more sensitive than human cells. The dominant binding motifs of Eu(III) in cell culture medium as well as cell suspensions are (organo-) phosphate groups. Additionally, a protein complex is formed in medium at low Eu(III) concentration. In contrast, U(VI) forms a carbonate complex in cell culture medium as well as each one phosphate and carbonate complex in cell suspensions. Using chemical microscopy, Eu(III) was localized in granular, vesicular compartments near the nucleus and the intracellular Eu(III) species equals the one in cell suspensions. Overall, this study contributes to a better understanding of the interactions of Ba(II), Eu(III), and U(VI) on a cellular and molecular level. Since Ba(II) and Eu(III) serve as inactive analogs of the radioactive Ra(II) and Am(III)/Cm(III), the results of this study are also of importance for the health risk assessment of these radionuclides.

AB - Heavy metals pose a potential health risk to humans when they enter the organism. Renal excretion is one of the elimination pathways and, therefore, investigations with kidney cells are of particular interest. In the present study, the effects of Ba(II), Eu(III), and U(VI) on rat and human renal cells were investigated in vitro. A combination of microscopic, biochemical, analytical, and spectroscopic methods was used to assess cell viability, cell death mechanisms, and intracellular metal uptake of exposed cells as well as metal speciation in cell culture medium and inside cells. For Eu(III) and U(VI), cytotoxicity and intracellular uptake are positively correlated and depend on concentration and exposure time. An enhanced apoptosis occurs upon Eu(III) exposure whereas U(VI) exposure leads to enhanced apoptosis and (secondary) necrosis. In contrast to that, Ba(II) exhibits no cytotoxic effect at all and its intracellular uptake is time-independently very low. In general, both cell lines give similar results with rat cells being more sensitive than human cells. The dominant binding motifs of Eu(III) in cell culture medium as well as cell suspensions are (organo-) phosphate groups. Additionally, a protein complex is formed in medium at low Eu(III) concentration. In contrast, U(VI) forms a carbonate complex in cell culture medium as well as each one phosphate and carbonate complex in cell suspensions. Using chemical microscopy, Eu(III) was localized in granular, vesicular compartments near the nucleus and the intracellular Eu(III) species equals the one in cell suspensions. Overall, this study contributes to a better understanding of the interactions of Ba(II), Eu(III), and U(VI) on a cellular and molecular level. Since Ba(II) and Eu(III) serve as inactive analogs of the radioactive Ra(II) and Am(III)/Cm(III), the results of this study are also of importance for the health risk assessment of these radionuclides.

KW - Chemical microscopy

KW - Cytotoxicity

KW - Heavy metal speciation

KW - Kidney cells

KW - Radionuclides

KW - TRLFS

UR - http://www.scopus.com/inward/record.url?scp=85187198197&partnerID=8YFLogxK

U2 - 10.1016/j.scitotenv.2024.171374

DO - 10.1016/j.scitotenv.2024.171374

M3 - Article

C2 - 38432374

AN - SCOPUS:85187198197

VL - 923

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 171374

ER -