Details
| Original language | English |
|---|---|
| Pages (from-to) | 471-485 |
| Number of pages | 15 |
| Journal | Plant molecular biology |
| Volume | 70 |
| Issue number | 4 |
| Publication status | Published - 27 Mar 2009 |
Abstract
Plant mitochondria include gamma-type carbonic anhydrases (γCAs) of unknown function. In Arabidopsis, the γCAs form a gene family of five members which all are attached to the NADH dehydrogenase complex (complex I) of the respiratory chain. Here we report a functional analysis of gamma carbonic anhydrase 2 (CA2). The gene encoding CA2 is constitutively expressed in all plant organs investigated but it is ten fold induced in flowers, particularly in tapetal tissue. Ectopic expression of CA2 in Arabidopsis causes male sterility in transgenic plants. In normal anther development, secondary thickenings of the endothecial cell wall cause anthers to open upon dehydration. Histological analyses revealed that abnormal secondary thickening prevents anther opening in 35S::CA2 transgenic plants. CA2 abundance in transgenic plants is increased 2-3 fold compared to wild-type plants as revealed by Western blotting analyses. Moreover, abundance of other members of the CA family, termed CA3 and CAL2, is increased in transgenic plants. Oxygen uptake measurements revealed that respiration in transgenic plants is mainly based on NADH reduction by the alternative NADH dehydrogenases present in plant mitochondria. Furthermore, the formation of reactive oxygen species (ROS) is very low in transgenic plants. We propose that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.
Keywords
- Anther, Arabidopsis thaliana, Gamma carbonic anhydrases, Mitochondria, Reactive oxigen species
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant molecular biology, Vol. 70, No. 4, 27.03.2009, p. 471-485.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Ectopic expression of mitochondrial gamma carbonic anhydrase 2 causes male sterility by anther indehiscence
AU - Villarreal, Fernando
AU - Martín, Victoria
AU - Colaneri, Alejandro
AU - González-Schain, Nahuel
AU - Perales, Mariano
AU - Martín, Mariana
AU - Lombardo, Cristina
AU - Braun, Hans Peter
AU - Bartoli, Carlos
AU - Zabaleta, Eduardo
N1 - Funding information: Acknowledgments This work was supported in part by ANPCyT (13432 and 31669) (Argentina), DAAD (Germany), ECOS-Sud, (A06B03) (France-Argentina). FV and MVM (CONICET, Argentina) are doctoral fellows and this work is part of their doctoral theses.
PY - 2009/3/27
Y1 - 2009/3/27
N2 - Plant mitochondria include gamma-type carbonic anhydrases (γCAs) of unknown function. In Arabidopsis, the γCAs form a gene family of five members which all are attached to the NADH dehydrogenase complex (complex I) of the respiratory chain. Here we report a functional analysis of gamma carbonic anhydrase 2 (CA2). The gene encoding CA2 is constitutively expressed in all plant organs investigated but it is ten fold induced in flowers, particularly in tapetal tissue. Ectopic expression of CA2 in Arabidopsis causes male sterility in transgenic plants. In normal anther development, secondary thickenings of the endothecial cell wall cause anthers to open upon dehydration. Histological analyses revealed that abnormal secondary thickening prevents anther opening in 35S::CA2 transgenic plants. CA2 abundance in transgenic plants is increased 2-3 fold compared to wild-type plants as revealed by Western blotting analyses. Moreover, abundance of other members of the CA family, termed CA3 and CAL2, is increased in transgenic plants. Oxygen uptake measurements revealed that respiration in transgenic plants is mainly based on NADH reduction by the alternative NADH dehydrogenases present in plant mitochondria. Furthermore, the formation of reactive oxygen species (ROS) is very low in transgenic plants. We propose that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.
AB - Plant mitochondria include gamma-type carbonic anhydrases (γCAs) of unknown function. In Arabidopsis, the γCAs form a gene family of five members which all are attached to the NADH dehydrogenase complex (complex I) of the respiratory chain. Here we report a functional analysis of gamma carbonic anhydrase 2 (CA2). The gene encoding CA2 is constitutively expressed in all plant organs investigated but it is ten fold induced in flowers, particularly in tapetal tissue. Ectopic expression of CA2 in Arabidopsis causes male sterility in transgenic plants. In normal anther development, secondary thickenings of the endothecial cell wall cause anthers to open upon dehydration. Histological analyses revealed that abnormal secondary thickening prevents anther opening in 35S::CA2 transgenic plants. CA2 abundance in transgenic plants is increased 2-3 fold compared to wild-type plants as revealed by Western blotting analyses. Moreover, abundance of other members of the CA family, termed CA3 and CAL2, is increased in transgenic plants. Oxygen uptake measurements revealed that respiration in transgenic plants is mainly based on NADH reduction by the alternative NADH dehydrogenases present in plant mitochondria. Furthermore, the formation of reactive oxygen species (ROS) is very low in transgenic plants. We propose that reduction in ROS inhibits H2O2 dependent lignin polymerization in CA2 over-expressing plants, thereby causing male sterility.
KW - Anther
KW - Arabidopsis thaliana
KW - Gamma carbonic anhydrases
KW - Mitochondria
KW - Reactive oxigen species
UR - http://www.scopus.com/inward/record.url?scp=67650594778&partnerID=8YFLogxK
U2 - 10.1007/s11103-009-9484-z
DO - 10.1007/s11103-009-9484-z
M3 - Article
C2 - 19326245
AN - SCOPUS:67650594778
VL - 70
SP - 471
EP - 485
JO - Plant molecular biology
JF - Plant molecular biology
SN - 0167-4412
IS - 4
ER -