Details
| Original language | English |
|---|---|
| Title of host publication | V International Postharvest Symposium |
| Publisher | International Society for Horticultural Science |
| Pages | 307-312 |
| Number of pages | 6 |
| ISBN (print) | 9789066056480 |
| Publication status | Published - 2005 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 682 |
| ISSN (Print) | 0567-7572 |
Abstract
Ornamental plants represent a high number of species from different plant families. To investigate the ethylene biosynthesis and isolate the genes involved in each species a fast and simple method is necessary. The enzyme that controls the ethylene synthesis is the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, which catalyses the formation of ACC, the immediate precursor of ethylene. Cloning of the first ACC synthase genes from different plant species revealed a multi-gene family.By using degenerate oligonucleotides that correspond to conserved amino acid residues of known ACC synthases, we amplified different genomic fragments that encodes ACC synthase homologue DNA fragments. As a result of the intron size variation of ACC synthase genes, the amplification of several ACC synthase genes with only one PCR reaction was possible. By means of this method we were able to isolate several unknown ACC synthase genes in a wide range of ornamental plant species such as Rosa hybrida, Euphorbia pulcherrima, Kalanchoe blossfeldiana, Pelargonium zonale and Campanula carpatica.
Keywords
- 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, Degenerate primer, Ethylene biosynthesis, Genomic sequences, Ornamental plants
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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V International Postharvest Symposium. International Society for Horticultural Science, 2005. p. 307-312 (Acta Horticulturae; Vol. 682).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Easy PCR method to isolate unknown ACC synthase genes in ornamental plant species
AU - Mibus, H.
AU - Serek, M.
PY - 2005
Y1 - 2005
N2 - Ornamental plants represent a high number of species from different plant families. To investigate the ethylene biosynthesis and isolate the genes involved in each species a fast and simple method is necessary. The enzyme that controls the ethylene synthesis is the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, which catalyses the formation of ACC, the immediate precursor of ethylene. Cloning of the first ACC synthase genes from different plant species revealed a multi-gene family.By using degenerate oligonucleotides that correspond to conserved amino acid residues of known ACC synthases, we amplified different genomic fragments that encodes ACC synthase homologue DNA fragments. As a result of the intron size variation of ACC synthase genes, the amplification of several ACC synthase genes with only one PCR reaction was possible. By means of this method we were able to isolate several unknown ACC synthase genes in a wide range of ornamental plant species such as Rosa hybrida, Euphorbia pulcherrima, Kalanchoe blossfeldiana, Pelargonium zonale and Campanula carpatica.
AB - Ornamental plants represent a high number of species from different plant families. To investigate the ethylene biosynthesis and isolate the genes involved in each species a fast and simple method is necessary. The enzyme that controls the ethylene synthesis is the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, which catalyses the formation of ACC, the immediate precursor of ethylene. Cloning of the first ACC synthase genes from different plant species revealed a multi-gene family.By using degenerate oligonucleotides that correspond to conserved amino acid residues of known ACC synthases, we amplified different genomic fragments that encodes ACC synthase homologue DNA fragments. As a result of the intron size variation of ACC synthase genes, the amplification of several ACC synthase genes with only one PCR reaction was possible. By means of this method we were able to isolate several unknown ACC synthase genes in a wide range of ornamental plant species such as Rosa hybrida, Euphorbia pulcherrima, Kalanchoe blossfeldiana, Pelargonium zonale and Campanula carpatica.
KW - 1-aminocyclopropane-1-carboxylic acid (ACC) synthase
KW - Degenerate primer
KW - Ethylene biosynthesis
KW - Genomic sequences
KW - Ornamental plants
UR - http://www.scopus.com/inward/record.url?scp=79952443702&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2005.682.34
DO - 10.17660/ActaHortic.2005.682.34
M3 - Conference contribution
AN - SCOPUS:79952443702
SN - 9789066056480
T3 - Acta Horticulturae
SP - 307
EP - 312
BT - V International Postharvest Symposium
PB - International Society for Horticultural Science
ER -