Details
| Original language | English |
|---|---|
| Pages (from-to) | 149-159 |
| Number of pages | 11 |
| Journal | PROTEOMICS |
| Volume | 8 |
| Issue number | 1 |
| Publication status | Published - 19 Dec 2007 |
Abstract
The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.) leaves. To unravel early Mn-toxicity responses after 1-3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn-sensitive cowpea cultivar. These proteins are involved in CO2 fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis-response reactions and protein degradation. Chloroplastic proteins important for CO2 fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn-sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn-stress response in cowpea.
Keywords
- Apoplast, Cowpea, Manganese toxicity, Symplast
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: PROTEOMICS, Vol. 8, No. 1, 19.12.2007, p. 149-159.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Early manganese-toxicity response in Vigna unguiculata L.
T2 - A proteomic and transcriptomic study
AU - Führs, Hendrik
AU - Hartwig, Moritz
AU - Molina, Laura Elisa Buitrago
AU - Heintz, Dimitri
AU - Van Dorsselaer, Alain
AU - Braun, Hans Peter
AU - Horst, Walter Johannes
PY - 2007/12/19
Y1 - 2007/12/19
N2 - The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.) leaves. To unravel early Mn-toxicity responses after 1-3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn-sensitive cowpea cultivar. These proteins are involved in CO2 fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis-response reactions and protein degradation. Chloroplastic proteins important for CO2 fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn-sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn-stress response in cowpea.
AB - The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.) leaves. To unravel early Mn-toxicity responses after 1-3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn-sensitive cowpea cultivar. These proteins are involved in CO2 fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis-response reactions and protein degradation. Chloroplastic proteins important for CO2 fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn-sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn-stress response in cowpea.
KW - Apoplast
KW - Cowpea
KW - Manganese toxicity
KW - Symplast
UR - http://www.scopus.com/inward/record.url?scp=38149040209&partnerID=8YFLogxK
U2 - 10.15488/11664
DO - 10.15488/11664
M3 - Article
C2 - 18095375
AN - SCOPUS:38149040209
VL - 8
SP - 149
EP - 159
JO - PROTEOMICS
JF - PROTEOMICS
SN - 1615-9853
IS - 1
ER -