Details
| Original language | English |
|---|---|
| Pages (from-to) | 688-696 |
| Number of pages | 9 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 18 |
| Issue number | 9 |
| Publication status | Published - 24 Apr 2012 |
| Externally published | Yes |
Abstract
Due to its biological significance, cell adhesion to biomaterial surfaces or scaffolds is the key step in biomedical applications. Here, we describe two sensitive and facile methods that quantify the kinetic and mechanic properties of the entire cell attachment process characterized by two parameters: Adhesion Time TAd and Adhesion Force FAd. We demonstrate that both methods can be applied to any adherent cell type (e.g., stem or cancer cells), tissue-engineered substrate, and culture condition in a fast, effective, and reproducible manner. Additional investigations about the role of the extracellular matrix and the formation of focal contacts help in acquiring further interpretations of these parameters from biological and mechanical points of view.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Engineering(all)
- Biomedical Engineering
Sustainable Development Goals
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In: Tissue Engineering - Part C: Methods, Vol. 18, No. 9, 24.04.2012, p. 688-696.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dynamics of Cell Attachment
T2 - Adhesion Time and Force
AU - Schlie, Sabrina
AU - Gruene, Martin
AU - Dittmar, Hagen
AU - Chichkov, Boris N.
PY - 2012/4/24
Y1 - 2012/4/24
N2 - Due to its biological significance, cell adhesion to biomaterial surfaces or scaffolds is the key step in biomedical applications. Here, we describe two sensitive and facile methods that quantify the kinetic and mechanic properties of the entire cell attachment process characterized by two parameters: Adhesion Time TAd and Adhesion Force FAd. We demonstrate that both methods can be applied to any adherent cell type (e.g., stem or cancer cells), tissue-engineered substrate, and culture condition in a fast, effective, and reproducible manner. Additional investigations about the role of the extracellular matrix and the formation of focal contacts help in acquiring further interpretations of these parameters from biological and mechanical points of view.
AB - Due to its biological significance, cell adhesion to biomaterial surfaces or scaffolds is the key step in biomedical applications. Here, we describe two sensitive and facile methods that quantify the kinetic and mechanic properties of the entire cell attachment process characterized by two parameters: Adhesion Time TAd and Adhesion Force FAd. We demonstrate that both methods can be applied to any adherent cell type (e.g., stem or cancer cells), tissue-engineered substrate, and culture condition in a fast, effective, and reproducible manner. Additional investigations about the role of the extracellular matrix and the formation of focal contacts help in acquiring further interpretations of these parameters from biological and mechanical points of view.
UR - http://www.scopus.com/inward/record.url?scp=84866983791&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2011.0635
DO - 10.1089/ten.tec.2011.0635
M3 - Article
C2 - 22429453
AN - SCOPUS:84866983791
VL - 18
SP - 688
EP - 696
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 9
ER -