Details
| Original language | English |
|---|---|
| Article number | 48 |
| Number of pages | 15 |
| Journal | Bioengineering |
| Volume | 5 |
| Issue number | 2 |
| Publication status | Published - 19 Jun 2018 |
| Externally published | Yes |
Abstract
Mesenchymal stem cells (MSCs) are considered as primary candidates for cell-based therapies due to their multiple effects in regenerative medicine. Pre-conditioning of MSCs under physiological conditions—such as hypoxia, three-dimensional environments, and dynamic cultivation—prior to transplantation proved to optimize their therapeutic efficiency. When cultivated as three-dimensional aggregates or spheroids, MSCs display increased angiogenic, anti-inflammatory, and immunomodulatory effects as well as improved stemness and survival rates after transplantation, and cultivation under dynamic conditions can increase their viability, proliferation, and paracrine effects, alike. Only few studies reported to date, however, have utilized dynamic conditions for three-dimensional aggregate cultivation of MSCs. Still, the integration of dynamic bioreactor systems, such as spinner flasks or stirred tank reactors might pave the way for a robust, scalable bulk expansion of MSC aggregates or MSC-derived extracellular vesicles. This review summarizes recent insights into the therapeutic potential of MSC aggregate cultivation and focuses on dynamic generation and cultivation techniques of MSC aggregates.
Keywords
- Aggregates, Bioreactor cultivation, Dynamic cultivation, Extracellular vesicles, Mesenchymal stem cells, Scaffold-free, Spheroids, Therapeutic potential
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
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In: Bioengineering, Vol. 5, No. 2, 48, 19.06.2018.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Dynamic Cultivation of Mesenchymal Stem Cell Aggregates
AU - Egger, Dominik
AU - Tripisciano, Carla
AU - Weber, Viktoria
AU - Dominici, Massimo
AU - Kasper, Cornelia
N1 - Funding Information: Funding: C.T. and V.W. received funding from the Christian Doppler Society (Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis).
PY - 2018/6/19
Y1 - 2018/6/19
N2 - Mesenchymal stem cells (MSCs) are considered as primary candidates for cell-based therapies due to their multiple effects in regenerative medicine. Pre-conditioning of MSCs under physiological conditions—such as hypoxia, three-dimensional environments, and dynamic cultivation—prior to transplantation proved to optimize their therapeutic efficiency. When cultivated as three-dimensional aggregates or spheroids, MSCs display increased angiogenic, anti-inflammatory, and immunomodulatory effects as well as improved stemness and survival rates after transplantation, and cultivation under dynamic conditions can increase their viability, proliferation, and paracrine effects, alike. Only few studies reported to date, however, have utilized dynamic conditions for three-dimensional aggregate cultivation of MSCs. Still, the integration of dynamic bioreactor systems, such as spinner flasks or stirred tank reactors might pave the way for a robust, scalable bulk expansion of MSC aggregates or MSC-derived extracellular vesicles. This review summarizes recent insights into the therapeutic potential of MSC aggregate cultivation and focuses on dynamic generation and cultivation techniques of MSC aggregates.
AB - Mesenchymal stem cells (MSCs) are considered as primary candidates for cell-based therapies due to their multiple effects in regenerative medicine. Pre-conditioning of MSCs under physiological conditions—such as hypoxia, three-dimensional environments, and dynamic cultivation—prior to transplantation proved to optimize their therapeutic efficiency. When cultivated as three-dimensional aggregates or spheroids, MSCs display increased angiogenic, anti-inflammatory, and immunomodulatory effects as well as improved stemness and survival rates after transplantation, and cultivation under dynamic conditions can increase their viability, proliferation, and paracrine effects, alike. Only few studies reported to date, however, have utilized dynamic conditions for three-dimensional aggregate cultivation of MSCs. Still, the integration of dynamic bioreactor systems, such as spinner flasks or stirred tank reactors might pave the way for a robust, scalable bulk expansion of MSC aggregates or MSC-derived extracellular vesicles. This review summarizes recent insights into the therapeutic potential of MSC aggregate cultivation and focuses on dynamic generation and cultivation techniques of MSC aggregates.
KW - Aggregates
KW - Bioreactor cultivation
KW - Dynamic cultivation
KW - Extracellular vesicles
KW - Mesenchymal stem cells
KW - Scaffold-free
KW - Spheroids
KW - Therapeutic potential
UR - http://www.scopus.com/inward/record.url?scp=85056765050&partnerID=8YFLogxK
U2 - 10.3390/bioengineering5020048
DO - 10.3390/bioengineering5020048
M3 - Review article
AN - SCOPUS:85056765050
VL - 5
JO - Bioengineering
JF - Bioengineering
IS - 2
M1 - 48
ER -