Details
| Original language | English |
|---|---|
| Pages (from-to) | 565-569 |
| Number of pages | 5 |
| Journal | Theoretical And Applied Genetics |
| Volume | 76 |
| Issue number | 4 |
| Publication status | Published - Oct 1988 |
| Externally published | Yes |
Abstract
Mitochondrial DNA (mtDNA) and transcriptional patterns of mitochondrial genes have been examined in dwarf, normal, fertile and male sterile Epilobium hybrids. No alterations or rearrangements of mitochondrial DNA could be detected in the developmentally disturbed hybrids. They exhibit restriction patterns of mtDNA that correspond exactly to those of their female parents. However, the transcription of at least one mitochondrial gene is significantly altered in the male sterile hybrid E. hirsutum x montanum. In normal plants, one mRNA of 1.6 kb hybridizes to the cytochrome c oxidase subunit II gene, while in male sterile plants a transcript of this size is lacking and instead a major transcript of 2.0 kb and two smaller ones occur. The transcript pattern of the F1 ATPase alpha subunit (atpA) gene exhibits slight alterations in sterile plants also. Since these hybrids have the same cytoplasm as normal plants, an incompatibility between the nuclear and the mitochondrial genotype may be responsible for the altered mitochondrial gene expression. No alteration of the transcripts of the mitochondrial genes tested could be detected in dwarf hybrids. The coincidence of male sterility with an altered transcription pattern of mitochondrial genes suggests that the mitochondria are involved in the occurrence of this phenotype.
Keywords
- Dwarfism, Epilobium, Gene expression, Male sterility, Mitochondrial DNA
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Theoretical And Applied Genetics, Vol. 76, No. 4, 10.1988, p. 565-569.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dwarfism and male sterility in interspecific hybrids of Epilobium
T2 - 2. Expression of mitochondrial genes and structure of the mitochondrial DNA
AU - Schmitz, Udo
AU - Michaelis, G.
PY - 1988/10
Y1 - 1988/10
N2 - Mitochondrial DNA (mtDNA) and transcriptional patterns of mitochondrial genes have been examined in dwarf, normal, fertile and male sterile Epilobium hybrids. No alterations or rearrangements of mitochondrial DNA could be detected in the developmentally disturbed hybrids. They exhibit restriction patterns of mtDNA that correspond exactly to those of their female parents. However, the transcription of at least one mitochondrial gene is significantly altered in the male sterile hybrid E. hirsutum x montanum. In normal plants, one mRNA of 1.6 kb hybridizes to the cytochrome c oxidase subunit II gene, while in male sterile plants a transcript of this size is lacking and instead a major transcript of 2.0 kb and two smaller ones occur. The transcript pattern of the F1 ATPase alpha subunit (atpA) gene exhibits slight alterations in sterile plants also. Since these hybrids have the same cytoplasm as normal plants, an incompatibility between the nuclear and the mitochondrial genotype may be responsible for the altered mitochondrial gene expression. No alteration of the transcripts of the mitochondrial genes tested could be detected in dwarf hybrids. The coincidence of male sterility with an altered transcription pattern of mitochondrial genes suggests that the mitochondria are involved in the occurrence of this phenotype.
AB - Mitochondrial DNA (mtDNA) and transcriptional patterns of mitochondrial genes have been examined in dwarf, normal, fertile and male sterile Epilobium hybrids. No alterations or rearrangements of mitochondrial DNA could be detected in the developmentally disturbed hybrids. They exhibit restriction patterns of mtDNA that correspond exactly to those of their female parents. However, the transcription of at least one mitochondrial gene is significantly altered in the male sterile hybrid E. hirsutum x montanum. In normal plants, one mRNA of 1.6 kb hybridizes to the cytochrome c oxidase subunit II gene, while in male sterile plants a transcript of this size is lacking and instead a major transcript of 2.0 kb and two smaller ones occur. The transcript pattern of the F1 ATPase alpha subunit (atpA) gene exhibits slight alterations in sterile plants also. Since these hybrids have the same cytoplasm as normal plants, an incompatibility between the nuclear and the mitochondrial genotype may be responsible for the altered mitochondrial gene expression. No alteration of the transcripts of the mitochondrial genes tested could be detected in dwarf hybrids. The coincidence of male sterility with an altered transcription pattern of mitochondrial genes suggests that the mitochondria are involved in the occurrence of this phenotype.
KW - Dwarfism
KW - Epilobium
KW - Gene expression
KW - Male sterility
KW - Mitochondrial DNA
UR - http://www.scopus.com/inward/record.url?scp=0000663943&partnerID=8YFLogxK
U2 - 10.1007/BF00260909
DO - 10.1007/BF00260909
M3 - Article
AN - SCOPUS:0000663943
VL - 76
SP - 565
EP - 569
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 4
ER -