Details
| Original language | English |
|---|---|
| Pages (from-to) | 140-148 |
| Number of pages | 9 |
| Journal | Engineering in life sciences |
| Volume | 13 |
| Issue number | 2 |
| Publication status | Published - 30 Aug 2012 |
Abstract
Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain in a 10-L bioreactor in batch and fed-batch mode, respectively. A new downstream process for polySia is presented, based on membrane adsorber technology and use of inorganic anion exchanger. These methods enable the replacement of precipitation steps, such as acetone, cetavlon, and ethanol precipitation of the already established purification process. The purification process was simplified, while process efficiency and product qualities were improved. The overall yield of polySia from a 10-L batch cultivation process was 61% and for 10-L fed-batch cultivation process the yield was 40% with an overall purity of 98%. The endotoxin content was determined to be negligible (14 EU mg-1). The main advantage of this new downstream process is that polySia with high chain length of more than 130 degree of polymerization can be obtained. In fed-batch cultivation, chain lengths up to 160 degree of polymerization were obtained.
Keywords
- Clay mineral, Escherichia coli K1, Membrane adsorber technique, Polysialic acid, Tissue engineering
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
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In: Engineering in life sciences, Vol. 13, No. 2, 30.08.2012, p. 140-148.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering
AU - Bice, Ismet
AU - Celik, Hilal
AU - Wolff, Christoph
AU - Beutel, Sascha
AU - Zahid, Maria
AU - Hitzmann, Bernd
AU - Rinas, Ursula
AU - Kasper, Cornelia
AU - Gerardy-Schahn, Rita
AU - Scheper, Thomas
PY - 2012/8/30
Y1 - 2012/8/30
N2 - Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain in a 10-L bioreactor in batch and fed-batch mode, respectively. A new downstream process for polySia is presented, based on membrane adsorber technology and use of inorganic anion exchanger. These methods enable the replacement of precipitation steps, such as acetone, cetavlon, and ethanol precipitation of the already established purification process. The purification process was simplified, while process efficiency and product qualities were improved. The overall yield of polySia from a 10-L batch cultivation process was 61% and for 10-L fed-batch cultivation process the yield was 40% with an overall purity of 98%. The endotoxin content was determined to be negligible (14 EU mg-1). The main advantage of this new downstream process is that polySia with high chain length of more than 130 degree of polymerization can be obtained. In fed-batch cultivation, chain lengths up to 160 degree of polymerization were obtained.
AB - Polysialic acid (polySia) is a carbohydrate polymer of varying chain length. It is a promising scaffold material for tissue engineering. In this work, high chain length polySia was produced by an Escherichia coli K1 strain in a 10-L bioreactor in batch and fed-batch mode, respectively. A new downstream process for polySia is presented, based on membrane adsorber technology and use of inorganic anion exchanger. These methods enable the replacement of precipitation steps, such as acetone, cetavlon, and ethanol precipitation of the already established purification process. The purification process was simplified, while process efficiency and product qualities were improved. The overall yield of polySia from a 10-L batch cultivation process was 61% and for 10-L fed-batch cultivation process the yield was 40% with an overall purity of 98%. The endotoxin content was determined to be negligible (14 EU mg-1). The main advantage of this new downstream process is that polySia with high chain length of more than 130 degree of polymerization can be obtained. In fed-batch cultivation, chain lengths up to 160 degree of polymerization were obtained.
KW - Clay mineral
KW - Escherichia coli K1
KW - Membrane adsorber technique
KW - Polysialic acid
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=84875009796&partnerID=8YFLogxK
U2 - 10.1002/elsc.201200041
DO - 10.1002/elsc.201200041
M3 - Article
AN - SCOPUS:84875009796
VL - 13
SP - 140
EP - 148
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 2
ER -