Details
| Original language | English |
|---|---|
| Title of host publication | Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI |
| Editors | Israel Gannot, Katy Roodenko |
| Place of Publication | Bellingham |
| Publisher | SPIE |
| Number of pages | 8 |
| ISBN (electronic) | 9781510641068 |
| ISBN (print) | 9781510641051 |
| Publication status | Published - 5 Mar 2021 |
| Event | Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI 2021 - Online, United States Duration: 6 Mar 2021 → 11 Mar 2021 |
Publication series
| Name | Proceedings of SPIE |
|---|---|
| Volume | 11635 |
| Name | Progress in biomedical optics and imaging |
|---|---|
| Publisher | SPIE |
| Number | 27 |
| Volume | 22 |
| ISSN (Print) | 1605-7422 |
Abstract
The US Food and Drug Administration (FDA) has high regulatory and quality requirements for the development and more importantly production of pharmaceuticals. Ideally, the production process is continuously monitored and recorded noninvasively to allow immediate control action if necessary. There are a multitude of established optical detection systems for classical stainless steel bioreactors using fiber optic probes, light guides, light sources and detectors for absorbance, fluorescence and Raman detection techniques. These systems are steam-sterilizable in place, reusable but are complex and expensive. Such systems are not well suited to use on the growing market of pre-sterilized disposable bioreactors, since there is a lack of sterile interface technology. A novel disposable and pre-sterilized flow cell has been developed, which can be easily connected to disposable bioreactors by sterile tube connections. The disposable BioFlowCell is clamped into a reusable flow cell holder, which is equipped with light guides, a light source and a detection unit. By this modular construction, spectroscopic techniques, like UV/Vis, fluorescence, turbidity by scattered light, near infrared or Raman spectroscopy are now usable for modern disposable bioreactors. We report on the suitability for in-line monitoring of mammalian cell cultures by detecting optical absorbance at 260, 280, 340 and 450 nm wavelengths to study aromatic amino acids, NADH and flavins simultaneously in a BioFlowCell with multiple pathlengths. Further, we studied in a second optical flowcell design, if light scattering and absorption in turbid samples can be used for biomass concentration measurements, as well as size or the viability of cells during continuous bioprocess experiment lasting several days.
Keywords
- Bioreactor, Disposable flowcell, In-line monitoring, Scattering, UV/VIS
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- Apa
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- BibTeX
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Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI. ed. / Israel Gannot; Katy Roodenko. Bellingham: SPIE, 2021. 1163514 (Proceedings of SPIE; Vol. 11635), (Progress in biomedical optics and imaging; Vol. 22, No. 27).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Disposable flowcell for spectroscopic analysis in bioprocesses
AU - Raithel, Philipp
AU - Steinwedel, Tobias
AU - Belz, Mathias
AU - Solle, Dörte
PY - 2021/3/5
Y1 - 2021/3/5
N2 - The US Food and Drug Administration (FDA) has high regulatory and quality requirements for the development and more importantly production of pharmaceuticals. Ideally, the production process is continuously monitored and recorded noninvasively to allow immediate control action if necessary. There are a multitude of established optical detection systems for classical stainless steel bioreactors using fiber optic probes, light guides, light sources and detectors for absorbance, fluorescence and Raman detection techniques. These systems are steam-sterilizable in place, reusable but are complex and expensive. Such systems are not well suited to use on the growing market of pre-sterilized disposable bioreactors, since there is a lack of sterile interface technology. A novel disposable and pre-sterilized flow cell has been developed, which can be easily connected to disposable bioreactors by sterile tube connections. The disposable BioFlowCell is clamped into a reusable flow cell holder, which is equipped with light guides, a light source and a detection unit. By this modular construction, spectroscopic techniques, like UV/Vis, fluorescence, turbidity by scattered light, near infrared or Raman spectroscopy are now usable for modern disposable bioreactors. We report on the suitability for in-line monitoring of mammalian cell cultures by detecting optical absorbance at 260, 280, 340 and 450 nm wavelengths to study aromatic amino acids, NADH and flavins simultaneously in a BioFlowCell with multiple pathlengths. Further, we studied in a second optical flowcell design, if light scattering and absorption in turbid samples can be used for biomass concentration measurements, as well as size or the viability of cells during continuous bioprocess experiment lasting several days.
AB - The US Food and Drug Administration (FDA) has high regulatory and quality requirements for the development and more importantly production of pharmaceuticals. Ideally, the production process is continuously monitored and recorded noninvasively to allow immediate control action if necessary. There are a multitude of established optical detection systems for classical stainless steel bioreactors using fiber optic probes, light guides, light sources and detectors for absorbance, fluorescence and Raman detection techniques. These systems are steam-sterilizable in place, reusable but are complex and expensive. Such systems are not well suited to use on the growing market of pre-sterilized disposable bioreactors, since there is a lack of sterile interface technology. A novel disposable and pre-sterilized flow cell has been developed, which can be easily connected to disposable bioreactors by sterile tube connections. The disposable BioFlowCell is clamped into a reusable flow cell holder, which is equipped with light guides, a light source and a detection unit. By this modular construction, spectroscopic techniques, like UV/Vis, fluorescence, turbidity by scattered light, near infrared or Raman spectroscopy are now usable for modern disposable bioreactors. We report on the suitability for in-line monitoring of mammalian cell cultures by detecting optical absorbance at 260, 280, 340 and 450 nm wavelengths to study aromatic amino acids, NADH and flavins simultaneously in a BioFlowCell with multiple pathlengths. Further, we studied in a second optical flowcell design, if light scattering and absorption in turbid samples can be used for biomass concentration measurements, as well as size or the viability of cells during continuous bioprocess experiment lasting several days.
KW - Bioreactor
KW - Disposable flowcell
KW - In-line monitoring
KW - Scattering
KW - UV/VIS
UR - http://www.scopus.com/inward/record.url?scp=85103814464&partnerID=8YFLogxK
U2 - 10.1117/12.2586196
DO - 10.1117/12.2586196
M3 - Conference contribution
AN - SCOPUS:85103814464
SN - 9781510641051
T3 - Proceedings of SPIE
BT - Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI
A2 - Gannot, Israel
A2 - Roodenko, Katy
PB - SPIE
CY - Bellingham
T2 - Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI 2021
Y2 - 6 March 2021 through 11 March 2021
ER -