Details
| Original language | English |
|---|---|
| Article number | 19 |
| Journal | Biomedical engineering online |
| Volume | 10 |
| Issue number | 19 |
| Publication status | Published - 7 Mar 2011 |
| Externally published | Yes |
Abstract
Background: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities.Methods: Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted.Results: The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified.Conclusions: These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs.
ASJC Scopus subject areas
- Health Professions(all)
- Radiological and Ultrasound Technology
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Medicine(all)
- Radiology Nuclear Medicine and imaging
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In: Biomedical engineering online, Vol. 10, No. 19, 19, 07.03.2011.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting
AU - Gruene, Martin
AU - Unger, Claudia
AU - Koch, Lothar
AU - Deiwick, Andrea
AU - Chichkov, Boris
N1 - Funding information: This work is supported by funding from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) for the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy) and Sonderforschungsbereich Transregio 37 (Mikro-und Nanosysteme in der Medizin). We thank Dipl.-Ing. Soenke Wienecke and Dipl.-Ing. Florian Evertz at the Institute for Multiphase Processes, Leibniz Universitaet Hannover, for their kind assistance with the characterization of the hydrogels.
PY - 2011/3/7
Y1 - 2011/3/7
N2 - Background: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities.Methods: Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted.Results: The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified.Conclusions: These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs.
AB - Background: Laser-assisted bioprinting of multi-cellular replicates in accordance with CAD blueprint may substantially improve our understandings of fundamental aspects of 3 D cell-cell and cell-matrix interactions in vitro. For predictable printing results, a profound knowledge about effects of different processing parameters is essential for realisation of 3 D cell models with well-defined cell densities.Methods: Time-resolved imaging of the hydrogel jet dynamics and quantitative assessment of the dependence of printed droplet diameter on the process characteristics were conducted.Results: The existence of a counterjet was visualised, proving the bubble collapsing theory for the jet formation. Furthermore, by adjusting the viscosity and height of the applied hydrogel layer in combination with different laser pulse energies, the printing of volumes in the range of 10 to 7000 picolitres was demonstrated. Additionally, the relationship between the viscosity and the layer thickness at different laser pulse energies on the printed droplet volume was identified.Conclusions: These findings are essential for the advancement of laser-assisted bioprinting by enabling predictable printing results and the integration of computational methods in the generation of 3 D multi-cellular constructs.
UR - http://www.scopus.com/inward/record.url?scp=79952230133&partnerID=8YFLogxK
U2 - 10.1186/1475-925X-10-19
DO - 10.1186/1475-925X-10-19
M3 - Article
C2 - 21385332
AN - SCOPUS:79952230133
VL - 10
JO - Biomedical engineering online
JF - Biomedical engineering online
SN - 1475-925X
IS - 19
M1 - 19
ER -