Details
| Original language | English |
|---|---|
| Pages (from-to) | 873-880 |
| Number of pages | 8 |
| Journal | Biology and fertility of soils |
| Volume | 57 |
| Issue number | 6 |
| Early online date | 22 Jun 2021 |
| Publication status | Published - Aug 2021 |
Abstract
Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.
Keywords
- Ammonium-based fertilization, Methanotroph, Methylosarcina, pmoA, Wetland agriculture
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Soil Science
- Immunology and Microbiology(all)
- Microbiology
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In: Biology and fertility of soils, Vol. 57, No. 6, 08.2021, p. 873-880.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Discrepancy in exchangeable and soluble ammonium-induced effects on aerobic methane oxidation
T2 - a microcosm study of a paddy soil
AU - van Dijk, Hester
AU - Kaupper, Thomas
AU - Bothe, Clemens
AU - Lee, Hyo Jung
AU - Bodelier, Paul L. E.
AU - Horn, Marcus A.
AU - Ho, Adrian
N1 - Funding Information: Open Access funding enabled and organized by Projekt DEAL. TK and AH are financially supported by the Deutsche Forschungsgemeinschaft (grant no. HO6234/1–1). AH and MAH are also financially supported by the Leibniz Universität Hannover, Germany.
PY - 2021/8
Y1 - 2021/8
N2 - Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.
AB - Ammonium-induced stimulatory, inhibitory, and/or neutral effects on soil methane oxidation have been attributable to the ammonium concentration and mineral forms, confounded by other edaphic properties (e.g., pH, salinity), as well as the site-specific composition of the methanotrophic community. We hypothesize that this inconsistency may stem from the discrepancy in the cation adsorption capacity of the soil. We postulate that the effects of ammonium on the methanotrophic activity in soil are more accurately portrayed by relating methane uptake rates to the soluble ammonium (bioavailable), rather than the exchangeable (total) ammonium. To reduce adsorption (exchangeable) sites for ammonium in a paddy soil, two successive pre-incubation steps were introduced resulting in a 1000-fold soil dilution (soil enrichment), to be compared to a soil slurry (tenfold dilution) incubation. Ammonium was supplemented as NH 4Cl at 0.5–4.75gL −1 after pre-incubation. While NH 4Cl significantly stimulated the methanotrophic activity at all concentrations in the soil slurry incubation, methane uptake showed a dose-dependent effect in the soil enrichment. The trend in methane uptake could be explained by the soluble ammonium concentration, which was proportionate to the supplemented ammonium in the soil enrichment. In the soil slurry incubation, a fraction (36–63%) of the supplemented ammonium was determined to be adsorbed to the soil. Accordingly, Methylosarcina was found to predominate the methanotrophic community after the incubation, suggesting the relevance of this methanotroph at elevated ammonium levels (< 3.25gL −1 NH 4Cl). Collectively, our results showed that the soluble, rather than the exchangeable ammonium concentration, is relevant when determining the effects of ammonium on methane oxidation, but this does not exclude other (a)biotic factors concurrently influencing methanotrophic activity.
KW - Ammonium-based fertilization
KW - Methanotroph
KW - Methylosarcina
KW - pmoA
KW - Wetland agriculture
UR - http://www.scopus.com/inward/record.url?scp=85108646253&partnerID=8YFLogxK
U2 - 10.1007/s00374-021-01579-9
DO - 10.1007/s00374-021-01579-9
M3 - Article
VL - 57
SP - 873
EP - 880
JO - Biology and fertility of soils
JF - Biology and fertility of soils
SN - 0178-2762
IS - 6
ER -