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Dimethyl sulfoxide and ethylene glycol promote membrane phase change during cryopreservation

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Ralf Spindler
  • Willem F. Wolkers
  • Birgit Glasmacher

Research Organisations

Details

Original languageEnglish
Pages (from-to)148-157
Number of pages10
JournalCryo-letters
Volume32
Issue number2
Publication statusPublished - 1 Mar 2011

Abstract

Fourier transform infrared spectroscopy (FTIR) and cryomicroscopy were used to study the effects of dimethyl sulfoxide and ethylene glycol on cell pellets of human pulmonary microvascular endothelial cells during freezing from 4°C to -60°C at 1°C/min. FTIR analysis showed that membranes undergo a phase change in the presence of cryoprotective agents (CPAs) which was not observed in the absence of CPAs. Cryomicroscopy revealed the formation of intracellular ice and concomitant cell volume changes. Intracellular ice was detected in the majority of the cells both in the presence and absence of CPAs. Membrane phase changes were found to be most pronounced at intermediate concentrations of cryoprotective agents; for dimethyl sulfoxide at around 1 M and for ethylene glycol at around 1.5 M. At those concentrations cell survival after thawing exhibited a maximum. The results indicate that CPAs promote rather than prevent cell dehydration during freezing.

Keywords

    Cryomicroscopy, Cryopreservation, Dehydration, Dimethyl sulfoxide, Ethylene glycol, FTIR

ASJC Scopus subject areas

Cite this

Dimethyl sulfoxide and ethylene glycol promote membrane phase change during cryopreservation. / Spindler, Ralf; Wolkers, Willem F.; Glasmacher, Birgit.
In: Cryo-letters, Vol. 32, No. 2, 01.03.2011, p. 148-157.

Research output: Contribution to journalArticleResearchpeer review

Spindler, Ralf ; Wolkers, Willem F. ; Glasmacher, Birgit. / Dimethyl sulfoxide and ethylene glycol promote membrane phase change during cryopreservation. In: Cryo-letters. 2011 ; Vol. 32, No. 2. pp. 148-157.
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Download

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