Details
| Original language | English |
|---|---|
| Title of host publication | Difference Gel Electrophoresis (DIGE) |
| Subtitle of host publication | Methods and Protocols |
| Pages | 335-342 |
| Number of pages | 8 |
| Publication status | Published - 12 Jan 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 854 |
| ISSN (Print) | 1064-3745 |
Abstract
Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.
Keywords
- Difference gel electrophoresis, Phenol extraction, Plant cell disruption, Plant proteomics, Protein extraction
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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Difference Gel Electrophoresis (DIGE): Methods and Protocols. 2012. p. 335-342 (Methods in Molecular Biology; Vol. 854).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method
AU - Rode, Christina
AU - Winkelmann, Traud
AU - Braun, Hans Peter
AU - Colditz, Frank
PY - 2012/1/12
Y1 - 2012/1/12
N2 - Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.
AB - Two-dimensional difference gel electrophoresis is an invaluable technique for the analysis of plant proteomes. However, preparation of protein fractions from plant tissues is challenging due to the special features of plant cells: a robust cell wall, large vacuoles which often contain high concentrations of organic acids and a broad range of secondary metabolites like phenolic compounds and pigments. Therefore, protein preparation for difference gel electrophoresis (DIGE) analyses has to be adapted. Here, we describe both a phenolic protein extraction method for plant tissues and an adapted protocol for DIGE labeling of the generated fractions.
KW - Difference gel electrophoresis
KW - Phenol extraction
KW - Plant cell disruption
KW - Plant proteomics
KW - Protein extraction
UR - http://www.scopus.com/inward/record.url?scp=84858143873&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-573-2_23
DO - 10.1007/978-1-61779-573-2_23
M3 - Contribution to book/anthology
C2 - 22311771
AN - SCOPUS:84858143873
SN - 9781617795725
T3 - Methods in Molecular Biology
SP - 335
EP - 342
BT - Difference Gel Electrophoresis (DIGE)
ER -