Details
| Original language | English |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | Developmental and Comparative Immunology |
| Volume | 67 |
| Publication status | Published - 1 Feb 2017 |
Abstract
Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.
Keywords
- 2D-IEF/SDS/PAGE, Frankliniella occidentalis, Innate immunity, Mass spectrometry, Proteomics, Tomato spotted wilt virus
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Immunology
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Developmental and Comparative Immunology, Vol. 67, 01.02.2017, p. 1-7.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)
AU - Ogada, Pamella Akoth
AU - Kiirika, Leonard Muriithi
AU - Lorenz, Christin
AU - Senkler, Jennifer
AU - Braun, Hans Peter
AU - Poehling, Hans Michael
N1 - Funding information: This research was funded by German Research Foundation (DFG - Deutsche Forschungsgemeinschaft) Project number: 207/37-1 . We thank the Wageningen University, the Netherlands for the supply of F. occidentalis and the TSWV isolates, also the entire technical support team, departments: Phytomedicine and Proteomics, Leibniz Universität, Hannover.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.
AB - Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.
KW - 2D-IEF/SDS/PAGE
KW - Frankliniella occidentalis
KW - Innate immunity
KW - Mass spectrometry
KW - Proteomics
KW - Tomato spotted wilt virus
UR - http://www.scopus.com/inward/record.url?scp=84994204378&partnerID=8YFLogxK
U2 - 10.1016/j.dci.2016.10.013
DO - 10.1016/j.dci.2016.10.013
M3 - Article
C2 - 27810283
AN - SCOPUS:84994204378
VL - 67
SP - 1
EP - 7
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
ER -