Details
| Original language | English |
|---|---|
| Pages (from-to) | 325-335 |
| Number of pages | 11 |
| Journal | Ecotoxicology |
| Volume | 27 |
| Issue number | 3 |
| Early online date | 5 Feb 2018 |
| Publication status | Published - Apr 2018 |
Abstract
Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.
Keywords
- Chromium, Microarray, Phenylalanine ammonia-lyase, Rice
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Toxicology
- Environmental Science(all)
- Management, Monitoring, Policy and Law
- Environmental Science(all)
- Health, Toxicology and Mutagenesis
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In: Ecotoxicology, Vol. 27, No. 3, 04.2018, p. 325-335.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Differential expression of the PAL gene family in rice seedlings exposed to chromium by microarray analysis
AU - Yu, Xiao Zhang
AU - Fan, Wei Jia
AU - Lin, Yu Juan
AU - Zhang, Fei Fei
AU - Gupta, Dharmendra K.
N1 - © 2018, Springer Science Business Media, LLC, part of Springer Nature
PY - 2018/4
Y1 - 2018/4
N2 - Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.
AB - Phenylalanine ammonia-lyase (PAL) is one of the principle enzymes involved in plant’s secondary metabolism. Expression of individual isogene from the PAL gene family is variable with species of plants in responses to different stresses. In this study, transcriptome analysis of the PAL gene family in rice seedlings exposed to potassium chromate Cr(VI) or chromium nitrate Cr(III) was conducted using Agilent 44K rice microarray and real-time quantitative RT-PCR. Uptake and accumulation of both Cr species by rice seedlings and their effect on PAL activity were also determined. Three days of Cr exposure led to significant accumulation of Cr in plant tissues, but majority being in roots rather than shoots. Changes of PAL activities in rice tissues were evident from both Cr treatments. Individual isogene from the rice PAL gene family was expressed differentially in response to both Cr variants. Comparing gene expression between two Cr treatments, only osPAL2 and osPAL4 genes were expressed in similar patterns. Also, gene expression pattern was inconsistent in both plant tissues. Results indicated that expression of individual isoform from the rice PAL gene family is tissue, and stimulus specific under different Cr exposure, suggesting their different detoxification strategies for decreasing or eliminating Cr stresses.
KW - Chromium
KW - Microarray
KW - Phenylalanine ammonia-lyase
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=85044465234&partnerID=8YFLogxK
U2 - 10.1007/s10646-018-1897-5
DO - 10.1007/s10646-018-1897-5
M3 - Article
C2 - 29404866
AN - SCOPUS:85044465234
VL - 27
SP - 325
EP - 335
JO - Ecotoxicology
JF - Ecotoxicology
SN - 0963-9292
IS - 3
ER -