Details
| Original language | English |
|---|---|
| Pages (from-to) | 178-187 |
| Number of pages | 10 |
| Journal | Plant physiology and biochemistry |
| Volume | 45 |
| Issue number | 3-4 |
| Publication status | Published - 1 Mar 2007 |
Abstract
Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyse the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Arabidopsis thaliana 20 Str proteins have been identified and grouped according to sequence homology. To investigate their respective in vivo function, Arabidopsis plants were grown in sterile hydroponic cultures at different sulfate (50, 500, and 1500 μM) and phosphate (0.1 and 1 mM) concentrations, and in medium supplemented with 1 mM thiosulfate. Northern blot analysis revealed the differential expression of the Str investigated. Thiosulfate Str activity was significantly increased at low sulfate concentrations in the medium. The Str mRNA levels were highly dependent on the developmental stage of the Arabidopsis plants. The expression of most Str analysed increased with progressing plant age in parallel with increasing 3-mercaptopyruvate and thiosulfate Str activities. The Str investigated were differentially expressed in a light/dark cycle whereas Str enzyme activities were not affected by the light conditions. The results indicate that each Str is regulated in a different way and plays an individual specific role in the plant metabolism.
Keywords
- 3-Mercaptopyruvate, Arabidopsis, Nutrition, Thiosulfate
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant physiology and biochemistry, Vol. 45, No. 3-4, 01.03.2007, p. 178-187.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Differential expression of Arabidopsis sulfurtransferases under various growth conditions
AU - Bartels, Andrea
AU - Mock, Hans Peter
AU - Papenbrock, Jutta
N1 - Funding information: We thank Pamela von Trzebiatowski and Julia Volker for their excellent technical assistance and the gardeners for growing the plants. The work was financially supported by the Deutsche Forschungsgemeinschaft (PA 764/1-4, SCHM 307/15-3).
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyse the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Arabidopsis thaliana 20 Str proteins have been identified and grouped according to sequence homology. To investigate their respective in vivo function, Arabidopsis plants were grown in sterile hydroponic cultures at different sulfate (50, 500, and 1500 μM) and phosphate (0.1 and 1 mM) concentrations, and in medium supplemented with 1 mM thiosulfate. Northern blot analysis revealed the differential expression of the Str investigated. Thiosulfate Str activity was significantly increased at low sulfate concentrations in the medium. The Str mRNA levels were highly dependent on the developmental stage of the Arabidopsis plants. The expression of most Str analysed increased with progressing plant age in parallel with increasing 3-mercaptopyruvate and thiosulfate Str activities. The Str investigated were differentially expressed in a light/dark cycle whereas Str enzyme activities were not affected by the light conditions. The results indicate that each Str is regulated in a different way and plays an individual specific role in the plant metabolism.
AB - Sulfurtransferases (Str) comprise a group of enzymes widely distributed in archaea, eubacteria, and eukaryota which catalyse the transfer of a sulfur atom from suitable sulfur donors to nucleophilic sulfur acceptors. Neither the in vivo sulfur donors nor the acceptors of Str could be clearly identified in any of the organisms investigated so far. In Arabidopsis thaliana 20 Str proteins have been identified and grouped according to sequence homology. To investigate their respective in vivo function, Arabidopsis plants were grown in sterile hydroponic cultures at different sulfate (50, 500, and 1500 μM) and phosphate (0.1 and 1 mM) concentrations, and in medium supplemented with 1 mM thiosulfate. Northern blot analysis revealed the differential expression of the Str investigated. Thiosulfate Str activity was significantly increased at low sulfate concentrations in the medium. The Str mRNA levels were highly dependent on the developmental stage of the Arabidopsis plants. The expression of most Str analysed increased with progressing plant age in parallel with increasing 3-mercaptopyruvate and thiosulfate Str activities. The Str investigated were differentially expressed in a light/dark cycle whereas Str enzyme activities were not affected by the light conditions. The results indicate that each Str is regulated in a different way and plays an individual specific role in the plant metabolism.
KW - 3-Mercaptopyruvate
KW - Arabidopsis
KW - Nutrition
KW - Thiosulfate
UR - http://www.scopus.com/inward/record.url?scp=34247349068&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2007.02.005
DO - 10.1016/j.plaphy.2007.02.005
M3 - Article
C2 - 17408957
AN - SCOPUS:34247349068
VL - 45
SP - 178
EP - 187
JO - Plant physiology and biochemistry
JF - Plant physiology and biochemistry
SN - 0981-9428
IS - 3-4
ER -