Details
| Original language | English |
|---|---|
| Pages (from-to) | 465-74 |
| Number of pages | 10 |
| Journal | Plant Journal |
| Volume | 53 |
| Issue number | 3 |
| Publication status | Published - Feb 2008 |
Abstract
We have investigated the establishment of histone H3 methylation with respect to environmental and developmental signals for two key genes associated with C4 photosynthesis in maize. Tri-methylation of histone H3 lysine 4 (H3K4) in roots and leaves was shown to be controlled by autonomous cell-type-specific developmental signals that are independent of illumination and therefore independent of the initiation of transcription. Di- and mono-methylation of H3K4 act antagonistically to this process. The modifications were already established in etiolated seedlings, and remained stable when genes were inactivated by dark treatment or pharmaceutical inhibition of transcription. Constitutive di-methylation of H3K9 was concomitantly detected at specific gene positions. The data support a histone code model whereby cell-type-specific signals induce the formation of a chromatin structure that potentiates gene activation by environmental cues.
Keywords
- Chromatin Immunoprecipitation, Epigenesis, Genetic, Genes, Plant/genetics, Histones/metabolism, Lysine/metabolism, Methylation, Photosynthesis/genetics, Plant Leaves/genetics, Polymerase Chain Reaction, Promoter Regions, Genetic/genetics, Zea mays/genetics
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In: Plant Journal, Vol. 53, No. 3, 02.2008, p. 465-74.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Developmental information but not promoter activity controls the methylation state of histone H3 lysine 4 on two photosynthetic genes in maize
AU - Danker, Tanja
AU - Dreesen, Björn
AU - Offermann, Sascha
AU - Horst, Ina
AU - Peterhänsel, Christoph
PY - 2008/2
Y1 - 2008/2
N2 - We have investigated the establishment of histone H3 methylation with respect to environmental and developmental signals for two key genes associated with C4 photosynthesis in maize. Tri-methylation of histone H3 lysine 4 (H3K4) in roots and leaves was shown to be controlled by autonomous cell-type-specific developmental signals that are independent of illumination and therefore independent of the initiation of transcription. Di- and mono-methylation of H3K4 act antagonistically to this process. The modifications were already established in etiolated seedlings, and remained stable when genes were inactivated by dark treatment or pharmaceutical inhibition of transcription. Constitutive di-methylation of H3K9 was concomitantly detected at specific gene positions. The data support a histone code model whereby cell-type-specific signals induce the formation of a chromatin structure that potentiates gene activation by environmental cues.
AB - We have investigated the establishment of histone H3 methylation with respect to environmental and developmental signals for two key genes associated with C4 photosynthesis in maize. Tri-methylation of histone H3 lysine 4 (H3K4) in roots and leaves was shown to be controlled by autonomous cell-type-specific developmental signals that are independent of illumination and therefore independent of the initiation of transcription. Di- and mono-methylation of H3K4 act antagonistically to this process. The modifications were already established in etiolated seedlings, and remained stable when genes were inactivated by dark treatment or pharmaceutical inhibition of transcription. Constitutive di-methylation of H3K9 was concomitantly detected at specific gene positions. The data support a histone code model whereby cell-type-specific signals induce the formation of a chromatin structure that potentiates gene activation by environmental cues.
KW - Chromatin Immunoprecipitation
KW - Epigenesis, Genetic
KW - Genes, Plant/genetics
KW - Histones/metabolism
KW - Lysine/metabolism
KW - Methylation
KW - Photosynthesis/genetics
KW - Plant Leaves/genetics
KW - Polymerase Chain Reaction
KW - Promoter Regions, Genetic/genetics
KW - Zea mays/genetics
U2 - 10.1111/j.1365-313X.2007.03352.x
DO - 10.1111/j.1365-313X.2007.03352.x
M3 - Article
C2 - 18179650
VL - 53
SP - 465
EP - 474
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 3
ER -