Details
| Original language | English |
|---|---|
| Title of host publication | VI International Symposium on Rose Research and Cultivation |
| Editors | T. Debener, M. Linde |
| Publisher | International Society for Horticultural Science |
| Pages | 177-184 |
| Number of pages | 8 |
| ISBN (electronic) | 9789462610552 |
| Publication status | Published - 25 Jan 2015 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 1064 |
| ISSN (Print) | 0567-7572 |
Abstract
Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.
Keywords
- Genotyping, Rosa hybrida, Rose, SNP, Tetraploid
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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VI International Symposium on Rose Research and Cultivation. ed. / T. Debener; M. Linde. International Society for Horticultural Science, 2015. p. 177-184 (Acta Horticulturae; Vol. 1064).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Development of the WagRhSNP AXIOM SNP array based on sequences from tetraploid cut roses and garden roses
AU - Smulders, M. J.M.
AU - Voorrips, R. E.
AU - Esselink, G. D.
AU - Santos Leonardo, T. M.
AU - Vant' Westende, W. P.C.
AU - Vukosavljev, M.
AU - Koning-Boucoiran, C. F.S.
AU - Van De Weg, W. E.
AU - Arens, P.
AU - Schulz, D.
AU - Debener, T.
AU - Bellon, L.
AU - Mittmann, M.
AU - Pirani, A.
AU - Webster, T.
AU - Brew, F.
AU - Cox, P.
AU - Maliepaard, C.
N1 - Publisher Copyright: © 2015, International Society for Horticultural Science. All rights reserved.
PY - 2015/1/25
Y1 - 2015/1/25
N2 - Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.
AB - Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.
KW - Genotyping
KW - Rosa hybrida
KW - Rose
KW - SNP
KW - Tetraploid
UR - http://www.scopus.com/inward/record.url?scp=84928109571&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2015.1064.20
DO - 10.17660/ActaHortic.2015.1064.20
M3 - Conference contribution
AN - SCOPUS:84928109571
T3 - Acta Horticulturae
SP - 177
EP - 184
BT - VI International Symposium on Rose Research and Cultivation
A2 - Debener, T.
A2 - Linde, M.
PB - International Society for Horticultural Science
ER -