Details
| Original language | English |
|---|---|
| Pages (from-to) | 219-224 |
| Number of pages | 6 |
| Journal | Gartenbauwissenschaft |
| Volume | 66 |
| Issue number | 5 |
| Publication status | Published - 2001 |
| Externally published | Yes |
Abstract
For differentiation of somatic embryos of Cyclamen persicum Mill. in liquid culture a protocol was developed regarding the parameters "fraction of the inoculum", "period of liquid culture", "plating density of globular embryos" and "fraction of the globular embryos plated". Moreover development of somatic embryos of cyclamen was performed in bioreactor culture which improved the yield of globular and germinating embryos in comparison to culture in flasks. Starting from one liter of a suspension culture in growth-regulator-free medium within 38 weeks a yield of 27,000 acclimatized young plants in the greenhouse was calculated.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Gartenbauwissenschaft, Vol. 66, No. 5, 2001, p. 219-224.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of somatic embryos of Cyclamen persicum Mill. in liquid culture
AU - Hohe, A.
AU - Winkelmann, T.
AU - Schwenkel, H. G.
PY - 2001
Y1 - 2001
N2 - For differentiation of somatic embryos of Cyclamen persicum Mill. in liquid culture a protocol was developed regarding the parameters "fraction of the inoculum", "period of liquid culture", "plating density of globular embryos" and "fraction of the globular embryos plated". Moreover development of somatic embryos of cyclamen was performed in bioreactor culture which improved the yield of globular and germinating embryos in comparison to culture in flasks. Starting from one liter of a suspension culture in growth-regulator-free medium within 38 weeks a yield of 27,000 acclimatized young plants in the greenhouse was calculated.
AB - For differentiation of somatic embryos of Cyclamen persicum Mill. in liquid culture a protocol was developed regarding the parameters "fraction of the inoculum", "period of liquid culture", "plating density of globular embryos" and "fraction of the globular embryos plated". Moreover development of somatic embryos of cyclamen was performed in bioreactor culture which improved the yield of globular and germinating embryos in comparison to culture in flasks. Starting from one liter of a suspension culture in growth-regulator-free medium within 38 weeks a yield of 27,000 acclimatized young plants in the greenhouse was calculated.
UR - http://www.scopus.com/inward/record.url?scp=0035677382&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0035677382
VL - 66
SP - 219
EP - 224
JO - Gartenbauwissenschaft
JF - Gartenbauwissenschaft
SN - 0016-478X
IS - 5
ER -