Details
| Original language | English |
|---|---|
| Pages (from-to) | 7-14 |
| Number of pages | 8 |
| Journal | Acta Horticulturae |
| Volume | 1232 |
| Publication status | Published - 1 Feb 2019 |
Abstract
Shoot organogenesis is an essential step for genetic engineering and the study of developmental processes in roses. Several adventitious shoot regeneration protocols have been established, but the number of responding cultivars is very limited. Furthermore, large differences in regeneration capacity between genotypes were reported rendering many cultivars as recalcitrant for shoot regeneration. Therefore, knowledge about the genetic complexity of the capacity for adventitious shoot regeneration and genes influencing this trait would be helpful for optimising regeneration protocols. Previously we analysed an association panel of 96 genotypes for in vitro shoot regeneration from petioles, and we detected a number of markers associated with the phenotypic traits. Here we present results of experiments to verify significantly associated markers in independent populations of garden roses using the KASP (Kompetitive Allele Specific PCR) technology.
Keywords
- In vitro, KASP assay, Rosa hybrida, Shoots regeneration, SNP markers
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Acta Horticulturae, Vol. 1232, 01.02.2019, p. 7-14.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of markers for shoot organogenesis in roses
AU - Nguyen, Thi Hong
AU - Winkelmann, Traud
AU - Debener, Thomas
N1 - Funding information: This work was carried out with financial support of the Department of Molecular Plant breeding, Institute of Plant Genetics, Leibniz Universität Hannover, Germany. We would like to acknowledge the Federal Plant Variety Office in Hannover for providing plant materials and Dr. Helgard Kaufmann for providing plant DNA materials
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Shoot organogenesis is an essential step for genetic engineering and the study of developmental processes in roses. Several adventitious shoot regeneration protocols have been established, but the number of responding cultivars is very limited. Furthermore, large differences in regeneration capacity between genotypes were reported rendering many cultivars as recalcitrant for shoot regeneration. Therefore, knowledge about the genetic complexity of the capacity for adventitious shoot regeneration and genes influencing this trait would be helpful for optimising regeneration protocols. Previously we analysed an association panel of 96 genotypes for in vitro shoot regeneration from petioles, and we detected a number of markers associated with the phenotypic traits. Here we present results of experiments to verify significantly associated markers in independent populations of garden roses using the KASP (Kompetitive Allele Specific PCR) technology.
AB - Shoot organogenesis is an essential step for genetic engineering and the study of developmental processes in roses. Several adventitious shoot regeneration protocols have been established, but the number of responding cultivars is very limited. Furthermore, large differences in regeneration capacity between genotypes were reported rendering many cultivars as recalcitrant for shoot regeneration. Therefore, knowledge about the genetic complexity of the capacity for adventitious shoot regeneration and genes influencing this trait would be helpful for optimising regeneration protocols. Previously we analysed an association panel of 96 genotypes for in vitro shoot regeneration from petioles, and we detected a number of markers associated with the phenotypic traits. Here we present results of experiments to verify significantly associated markers in independent populations of garden roses using the KASP (Kompetitive Allele Specific PCR) technology.
KW - In vitro
KW - KASP assay
KW - Rosa hybrida
KW - Shoots regeneration
KW - SNP markers
UR - http://www.scopus.com/inward/record.url?scp=85061495100&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2019.1232.2
DO - 10.17660/ActaHortic.2019.1232.2
M3 - Article
AN - SCOPUS:85061495100
VL - 1232
SP - 7
EP - 14
JO - Acta Horticulturae
JF - Acta Horticulturae
SN - 0567-7572
ER -