Details
| Original language | English |
|---|---|
| Pages (from-to) | 109-111 |
| Number of pages | 3 |
| Journal | Journal of biotechnology |
| Volume | 217 |
| Publication status | Published - 19 Nov 2015 |
Abstract
During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.
Keywords
- Living mammalian cell, Microarray technology, Piezoelectric nanoprinter
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Sustainable Development Goals
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In: Journal of biotechnology, Vol. 217, 19.11.2015, p. 109-111.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of living cell microarrays using non-contactmicropipette printing
AU - Jonczyk, Rebecca
AU - Timur, Suna
AU - Scheper, Thomas
AU - Stahl, Frank
N1 - Funding information: This work was supported by Federal Ministry of Education and Research (BMBF) and by Niedersächsische Krebsgesellschaft e.V . We are grateful to Dr. Antonina Lavrentieva and Dr. Johanna Walter for her critical reading of the manuscript. We further thank Paul Maschhoff for proofreading of the manuscript.
PY - 2015/11/19
Y1 - 2015/11/19
N2 - During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.
AB - During the last 30 years cellular screening systems were unidirectional developed towards high throughput applications on single cell level. We developed living cell microarrays, which provide an in vivo-like microenvironment for an advanced method to measure cellular response to external stimuli. To print living cells on glass slides, the classic microarray equipment, which involves printer and scanner, was fully transferred to suspensions of living cells. The microarray production was optimized using a contact-free spotting procedure in order to enhanced cell adhesion and growth rates. The printed model cells, A-549 (lung cancer cell line), were analyzed with conventional cell staining assays like DAPI (cell nuclei staining), calcein acetoxymethyl ester (viable cell staining), and CellTiter-Blue® Cell Viability Assay. After optimization, a reproducible (spot-to-spot variation:±8.6 cells) printing method for small living cell amounts (1200cells and fewer) was established that achieved cell viabilities of up to 88% for ≥0.6μL and good proliferation characteristics. Hence, this method could be advantageous for use in biomedical and diagnostic applications.
KW - Living mammalian cell
KW - Microarray technology
KW - Piezoelectric nanoprinter
UR - http://www.scopus.com/inward/record.url?scp=84949267070&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2015.11.013
DO - 10.1016/j.jbiotec.2015.11.013
M3 - Article
C2 - 26603124
AN - SCOPUS:84949267070
VL - 217
SP - 109
EP - 111
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
ER -