Details
| Original language | English |
|---|---|
| Title of host publication | VI International Conference Postharvest Unlimited |
| Editors | D. Valero, M. Serrano, M. Gil |
| Publisher | International Society for Horticultural Science |
| Pages | 107-113 |
| Number of pages | 7 |
| ISBN (electronic) | 978-94-62612-53-2 |
| Publication status | Published - 8 Nov 2019 |
| Event | VI International Conference Postharvest Unlimited - Madrid, Spain Duration: 17 Oct 2017 → 20 Oct 2017 |
Publication series
| Name | ISHS Acta Horticulturae |
|---|---|
| Publisher | International Society for Horticultural Science |
| Volume | 1256 |
| ISSN (Print) | 0567-7572 |
| ISSN (electronic) | 2406-6168 |
Abstract
Improvement of quality and display life of miniature potted roses, Rosa hybrida L., is an important issue for rose breeders. Sensitivity to ethylene significantly influences the postproduction life of different cultivars. Availability of molecular markers that in the early growth stages can distinguish between rose cultivars with high or low ethylene sensitivity would therefore be valuable. Two tetraploid miniature potted cultivars, i.e., ‘Vanilla’ which is only slightly sensitive to ethylene and ‘Lavender’ which is very sensitive to ethylene, as well as four ‘Vanilla’ × ‘Lavender’ F1 progeny plants of low sensitivity and four of high sensitivity were investigated. The genes for the ethylene receptor RhETR3 and the transcription factor RhEIN3 were selected for development of cleaved amplified polymorphic sequence (CAPS) markers. Amplified fragments of RhETR3 and RhEIN3 cDNA of ‘Vanilla’ and ‘Lavender’ were cloned, sequenced and subjected to digestion with restriction enzymes with recognition sites containing SNPs detected by comparison of the sequences from ‘Vanilla’ and ‘Lavender’. Two slightly different alleles of each gene were identified for both ‘Vanilla’ and ‘Lavender’. The SNPs located in restriction enzyme sites lead to the expected differences in restriction fragment sizes between ‘Vanilla’ and ‘Lavender’ cDNAs, but the results for the F1 progeny plants are not correlated with their ethylene sensitivity. Since the plants are tetraploid, characterization of more alleles of the RhETR3 and RhEIN3 genes and more SNPs may be required to obtain a useful system of markers.
Keywords
- Molecular marker, Postharvest, RhEIN3, RhERT3, Rose breeding, SNPs
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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VI International Conference Postharvest Unlimited. ed. / D. Valero; M. Serrano; M. Gil. International Society for Horticultural Science, 2019. p. 107-113 (ISHS Acta Horticulturae; Vol. 1256).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Development of cleaved amplified polymorphic sequence (CAPS) markers that may indicate ethylene sensitivity in miniature potted roses
AU - Al-Salem, Mohammad
AU - Serek, Margrethe
N1 - Funding information: The experimental work was partly supported by a Ph.D. grant from the German Academic Exchange Service (DAAD) (Ref.: 412, PKZ: A/03/33925). The authors would like to thank Prof. em. Bjarne M. Stummann (University of Copenhagen, Denmark) for valuable advices and critical review of the manuscript, and to thank the technical and scientific staff from the Section of Floriculture at Leibniz University of Hannover for fruitful discussions and technical assistance.
PY - 2019/11/8
Y1 - 2019/11/8
N2 - Improvement of quality and display life of miniature potted roses, Rosa hybrida L., is an important issue for rose breeders. Sensitivity to ethylene significantly influences the postproduction life of different cultivars. Availability of molecular markers that in the early growth stages can distinguish between rose cultivars with high or low ethylene sensitivity would therefore be valuable. Two tetraploid miniature potted cultivars, i.e., ‘Vanilla’ which is only slightly sensitive to ethylene and ‘Lavender’ which is very sensitive to ethylene, as well as four ‘Vanilla’ × ‘Lavender’ F1 progeny plants of low sensitivity and four of high sensitivity were investigated. The genes for the ethylene receptor RhETR3 and the transcription factor RhEIN3 were selected for development of cleaved amplified polymorphic sequence (CAPS) markers. Amplified fragments of RhETR3 and RhEIN3 cDNA of ‘Vanilla’ and ‘Lavender’ were cloned, sequenced and subjected to digestion with restriction enzymes with recognition sites containing SNPs detected by comparison of the sequences from ‘Vanilla’ and ‘Lavender’. Two slightly different alleles of each gene were identified for both ‘Vanilla’ and ‘Lavender’. The SNPs located in restriction enzyme sites lead to the expected differences in restriction fragment sizes between ‘Vanilla’ and ‘Lavender’ cDNAs, but the results for the F1 progeny plants are not correlated with their ethylene sensitivity. Since the plants are tetraploid, characterization of more alleles of the RhETR3 and RhEIN3 genes and more SNPs may be required to obtain a useful system of markers.
AB - Improvement of quality and display life of miniature potted roses, Rosa hybrida L., is an important issue for rose breeders. Sensitivity to ethylene significantly influences the postproduction life of different cultivars. Availability of molecular markers that in the early growth stages can distinguish between rose cultivars with high or low ethylene sensitivity would therefore be valuable. Two tetraploid miniature potted cultivars, i.e., ‘Vanilla’ which is only slightly sensitive to ethylene and ‘Lavender’ which is very sensitive to ethylene, as well as four ‘Vanilla’ × ‘Lavender’ F1 progeny plants of low sensitivity and four of high sensitivity were investigated. The genes for the ethylene receptor RhETR3 and the transcription factor RhEIN3 were selected for development of cleaved amplified polymorphic sequence (CAPS) markers. Amplified fragments of RhETR3 and RhEIN3 cDNA of ‘Vanilla’ and ‘Lavender’ were cloned, sequenced and subjected to digestion with restriction enzymes with recognition sites containing SNPs detected by comparison of the sequences from ‘Vanilla’ and ‘Lavender’. Two slightly different alleles of each gene were identified for both ‘Vanilla’ and ‘Lavender’. The SNPs located in restriction enzyme sites lead to the expected differences in restriction fragment sizes between ‘Vanilla’ and ‘Lavender’ cDNAs, but the results for the F1 progeny plants are not correlated with their ethylene sensitivity. Since the plants are tetraploid, characterization of more alleles of the RhETR3 and RhEIN3 genes and more SNPs may be required to obtain a useful system of markers.
KW - Molecular marker
KW - Postharvest
KW - RhEIN3
KW - RhERT3
KW - Rose breeding
KW - SNPs
UR - http://www.scopus.com/inward/record.url?scp=85075264246&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2019.1256.15
DO - 10.17660/ActaHortic.2019.1256.15
M3 - Conference contribution
AN - SCOPUS:85075264246
T3 - ISHS Acta Horticulturae
SP - 107
EP - 113
BT - VI International Conference Postharvest Unlimited
A2 - Valero, D.
A2 - Serrano, M.
A2 - Gil, M.
PB - International Society for Horticultural Science
T2 - VI International Conference Postharvest Unlimited
Y2 - 17 October 2017 through 20 October 2017
ER -