Details
| Original language | English |
|---|---|
| Pages (from-to) | 259-264 |
| Number of pages | 6 |
| Journal | Analytica chimica acta |
| Volume | 317 |
| Issue number | 1-3 |
| Publication status | Published - 20 Dec 1995 |
Abstract
Simple non-structured fluoride sensitive electrolyte isolator semiconductor (EIS) chips consisting of SiSiO2Si3N4 LaF3 layers were used as transducers for measuring the H2O2 concentration by peroxidasej (POD) and combined this H2O2 sensor with various oxidases, which form H2O2 in stoichiometric amount. The concentrations of the following analytes were measured with this system: glucose with glucose oxidase (GOD) and POD, maltose with amyloglucosidase (AGLU), mutarotase (MUT), GOD and POD, amylose with β-amylase (Amyl), AGLU, mut, GOD and POD, sucrose with invertase (INV), MUT, GOD and POD, ethanol with alcohol oxidase and POD, Sulfite with sulfiteoxidase (SOD) and POD, xanthine with xanthineoxidase (XAD) and POD, phosphate with nucleoside-phosphorylase (NP), XOD and POD. The required enzymes were coimmobilized on the surface of the transducer (pF-EIS-CAP), mounted into a flow cell, equipped with a reference electrode, integrated into a flow injection analyser (FIA) system and operated with the CAFCA (Computer Assisted Flow Control and Analysis) automation programme.
Keywords
- Biosensors, Flow injection, Process monitoring, Semiconductors
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 317, No. 1-3, 20.12.1995, p. 259-264.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of biosensors based on an electrolyte isolator semiconductor (EIS) -capacitor structure and their application for process monitoring. Part I. Development of the biosensors and their characterization
AU - Menzel, C.
AU - Lerch, T.
AU - Scheper, T.
AU - Schügerl, K.
N1 - Funding information: The authorsg ratefullya cknowledgteh e financial support from the German Scientific Foundation (DFG) and the Federal Ministry of Researcha nd Technology( BMFT ProjectN o. 0310087A)
PY - 1995/12/20
Y1 - 1995/12/20
N2 - Simple non-structured fluoride sensitive electrolyte isolator semiconductor (EIS) chips consisting of SiSiO2Si3N4 LaF3 layers were used as transducers for measuring the H2O2 concentration by peroxidasej (POD) and combined this H2O2 sensor with various oxidases, which form H2O2 in stoichiometric amount. The concentrations of the following analytes were measured with this system: glucose with glucose oxidase (GOD) and POD, maltose with amyloglucosidase (AGLU), mutarotase (MUT), GOD and POD, amylose with β-amylase (Amyl), AGLU, mut, GOD and POD, sucrose with invertase (INV), MUT, GOD and POD, ethanol with alcohol oxidase and POD, Sulfite with sulfiteoxidase (SOD) and POD, xanthine with xanthineoxidase (XAD) and POD, phosphate with nucleoside-phosphorylase (NP), XOD and POD. The required enzymes were coimmobilized on the surface of the transducer (pF-EIS-CAP), mounted into a flow cell, equipped with a reference electrode, integrated into a flow injection analyser (FIA) system and operated with the CAFCA (Computer Assisted Flow Control and Analysis) automation programme.
AB - Simple non-structured fluoride sensitive electrolyte isolator semiconductor (EIS) chips consisting of SiSiO2Si3N4 LaF3 layers were used as transducers for measuring the H2O2 concentration by peroxidasej (POD) and combined this H2O2 sensor with various oxidases, which form H2O2 in stoichiometric amount. The concentrations of the following analytes were measured with this system: glucose with glucose oxidase (GOD) and POD, maltose with amyloglucosidase (AGLU), mutarotase (MUT), GOD and POD, amylose with β-amylase (Amyl), AGLU, mut, GOD and POD, sucrose with invertase (INV), MUT, GOD and POD, ethanol with alcohol oxidase and POD, Sulfite with sulfiteoxidase (SOD) and POD, xanthine with xanthineoxidase (XAD) and POD, phosphate with nucleoside-phosphorylase (NP), XOD and POD. The required enzymes were coimmobilized on the surface of the transducer (pF-EIS-CAP), mounted into a flow cell, equipped with a reference electrode, integrated into a flow injection analyser (FIA) system and operated with the CAFCA (Computer Assisted Flow Control and Analysis) automation programme.
KW - Biosensors
KW - Flow injection
KW - Process monitoring
KW - Semiconductors
UR - http://www.scopus.com/inward/record.url?scp=0029585913&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(95)00419-X
DO - 10.1016/0003-2670(95)00419-X
M3 - Article
AN - SCOPUS:0029585913
VL - 317
SP - 259
EP - 264
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - 1-3
ER -