Details
| Original language | English |
|---|---|
| Article number | 124 |
| Journal | Biosensors |
| Volume | 9 |
| Issue number | 4 |
| Early online date | 14 Oct 2019 |
| Publication status | Published - Dec 2019 |
Abstract
Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high anity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like age-related macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGF-binding aptamers were investigated for the systematic development of a target-induced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers.
Keywords
- Aptamer, Biosensor, Microarray, Protein detection, Target-induced dissociation (TID), Thermophoresis, Vascular endothelial growth factor (VEGF)
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
Sustainable Development Goals
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In: Biosensors, Vol. 9, No. 4, 124, 12.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays
AU - Kurth, Tracy
AU - Witt, Sandra
AU - Bolten, Svenja
AU - Waniek, Janice Joy
AU - Kortmann, Carlotta
AU - Lavrentieva, Antonina
AU - Scheper, Thomas
AU - Walter, Johanna Gabriela
N1 - Funding information: Parts of this work were funded by German Research Foundation (DFG) and by the Max Buchner Research Foundation. The publication of this article was funded by the Open Access fund of Leibniz Universität Hannover.
PY - 2019/12
Y1 - 2019/12
N2 - Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high anity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like age-related macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGF-binding aptamers were investigated for the systematic development of a target-induced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers.
AB - Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high anity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like age-related macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGF-binding aptamers were investigated for the systematic development of a target-induced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers.
KW - Aptamer
KW - Biosensor
KW - Microarray
KW - Protein detection
KW - Target-induced dissociation (TID)
KW - Thermophoresis
KW - Vascular endothelial growth factor (VEGF)
UR - http://www.scopus.com/inward/record.url?scp=85073452049&partnerID=8YFLogxK
U2 - 10.3390/bios9040124
DO - 10.3390/bios9040124
M3 - Article
C2 - 31615077
AN - SCOPUS:85073452049
VL - 9
JO - Biosensors
JF - Biosensors
IS - 4
M1 - 124
ER -