Details
| Original language | English |
|---|---|
| Article number | 1700839 |
| Journal | Advanced healthcare materials |
| Volume | 7 |
| Issue number | 5 |
| Early online date | 29 Sept 2017 |
| Publication status | Published - 7 Mar 2018 |
| Externally published | Yes |
Abstract
Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.
Keywords
- antifouling surfaces, antimicrobial peptides, mussel-inspired chemistry, platelet and bacterial adhesion, polyvinyl chloride platelet storage bags
ASJC Scopus subject areas
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Biomedical Engineering
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
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In: Advanced healthcare materials, Vol. 7, No. 5, 1700839, 07.03.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of Antifouling and Bactericidal Coatings for Platelet Storage Bags Using Dopamine Chemistry
AU - Hadjesfandiari, Narges
AU - Weinhart, Marie
AU - Kizhakkedathu, Jayachandran N.
AU - Haag, Rainer
AU - Brooks, Donald E.
N1 - Funding information: The authors thank Dr. Sandra Ramirez-Arcos, Canadian Blood Services, Ottawa for providing the clinical bacterial strains. They also thank Dr. Tae Kyong John Kim, UBC, for collecting the TOF-SIMS data. N.H. thanks Dr. Silke Heinen and Dr. Qiang Wei, Freie Universität Berlin, for their input on QCM-D experiments. This work was supported by Natural Sciences and Engineering Research Council of Canada. The authors benefited from the facilities of the UBC Centre for Blood Research, Department of Chemistry and Department of Materials Engineering. N.H. is a recipient of a Health Canada/Canadian Blood Services graduate fellowship. J.N.K. is a recipient of a Michael Smith Foundation for Health Research Career Scholar Award.
PY - 2018/3/7
Y1 - 2018/3/7
N2 - Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.
AB - Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.
KW - antifouling surfaces
KW - antimicrobial peptides
KW - mussel-inspired chemistry
KW - platelet and bacterial adhesion
KW - polyvinyl chloride platelet storage bags
UR - http://www.scopus.com/inward/record.url?scp=85030330565&partnerID=8YFLogxK
U2 - 10.1002/adhm.201700839
DO - 10.1002/adhm.201700839
M3 - Article
C2 - 28961393
AN - SCOPUS:85030330565
VL - 7
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 5
M1 - 1700839
ER -