Details
| Original language | English |
|---|---|
| Pages (from-to) | 33-42 |
| Number of pages | 10 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 80 |
| Issue number | 1 |
| Publication status | Published - Jan 2005 |
Abstract
The attempts of this investigation were to develop a system for plant regeneration from explants of adult plants and its use for genetic transformation of important commercial Pelargonium zonale hybrid and P. peltatum hybrid cultivars. To this aim, leaf blade and petiole explants of eight cultivars were cultured on modified MS (Murashige and Skoog, 1962) medium with two concentrations of TDZ, BA, and zeatin (5 and 20 μM). Petiole explants showed a higher regeneration response than leaf blade explants and TDZ was the most effective cytokinin. Petioles of 16 cultivars were incubated on medium containing 5, 10, 15, and 20 μM TDZ, respectively, in order to identify the most effective TDZ concentration. For the majority of genotypes 10 μM TDZ resulted in the best regeneration response. Cefotaxim at 500 mg l-1 had no effect on shoot regeneration and did not show interaction with glufosinate. For selection of transgenic cells, a concentration of 2.5 μM glufosinate was shown to be appropriate. LBA4404 and EHA101 Agrobacterium strains carrying pIBGUS vector with pat gene as selectable marker gene and GUS gene as reporter gene were compared in transformation studies. With regard to GUS expression in petiole explants 16 days after coculture, better results were obtained with EHA 101 than with LBA 4404.
Keywords
- Agrobacterium-mediated gene transfer, Breeding, Geranium, In vitro culture, Pelargonium x hortorum, Regeneration ability
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Plant Cell, Tissue and Organ Culture, Vol. 80, No. 1, 01.2005, p. 33-42.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Development of a shoot regeneration protocol for genetic transformation in Pelargonium zonale and Pelargonium peltatum hybrids
AU - Winkelmann, Traud
AU - Kaviani, Kamran
AU - Serek, Margrethe
PY - 2005/1
Y1 - 2005/1
N2 - The attempts of this investigation were to develop a system for plant regeneration from explants of adult plants and its use for genetic transformation of important commercial Pelargonium zonale hybrid and P. peltatum hybrid cultivars. To this aim, leaf blade and petiole explants of eight cultivars were cultured on modified MS (Murashige and Skoog, 1962) medium with two concentrations of TDZ, BA, and zeatin (5 and 20 μM). Petiole explants showed a higher regeneration response than leaf blade explants and TDZ was the most effective cytokinin. Petioles of 16 cultivars were incubated on medium containing 5, 10, 15, and 20 μM TDZ, respectively, in order to identify the most effective TDZ concentration. For the majority of genotypes 10 μM TDZ resulted in the best regeneration response. Cefotaxim at 500 mg l-1 had no effect on shoot regeneration and did not show interaction with glufosinate. For selection of transgenic cells, a concentration of 2.5 μM glufosinate was shown to be appropriate. LBA4404 and EHA101 Agrobacterium strains carrying pIBGUS vector with pat gene as selectable marker gene and GUS gene as reporter gene were compared in transformation studies. With regard to GUS expression in petiole explants 16 days after coculture, better results were obtained with EHA 101 than with LBA 4404.
AB - The attempts of this investigation were to develop a system for plant regeneration from explants of adult plants and its use for genetic transformation of important commercial Pelargonium zonale hybrid and P. peltatum hybrid cultivars. To this aim, leaf blade and petiole explants of eight cultivars were cultured on modified MS (Murashige and Skoog, 1962) medium with two concentrations of TDZ, BA, and zeatin (5 and 20 μM). Petiole explants showed a higher regeneration response than leaf blade explants and TDZ was the most effective cytokinin. Petioles of 16 cultivars were incubated on medium containing 5, 10, 15, and 20 μM TDZ, respectively, in order to identify the most effective TDZ concentration. For the majority of genotypes 10 μM TDZ resulted in the best regeneration response. Cefotaxim at 500 mg l-1 had no effect on shoot regeneration and did not show interaction with glufosinate. For selection of transgenic cells, a concentration of 2.5 μM glufosinate was shown to be appropriate. LBA4404 and EHA101 Agrobacterium strains carrying pIBGUS vector with pat gene as selectable marker gene and GUS gene as reporter gene were compared in transformation studies. With regard to GUS expression in petiole explants 16 days after coculture, better results were obtained with EHA 101 than with LBA 4404.
KW - Agrobacterium-mediated gene transfer
KW - Breeding
KW - Geranium
KW - In vitro culture
KW - Pelargonium x hortorum
KW - Regeneration ability
UR - http://www.scopus.com/inward/record.url?scp=17644381995&partnerID=8YFLogxK
U2 - 10.1007/s11240-004-5788-5
DO - 10.1007/s11240-004-5788-5
M3 - Article
AN - SCOPUS:17644381995
VL - 80
SP - 33
EP - 42
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -